Multinucleate giant cells and the control of chemokine secretion in response to Mycobacterium tuberculosis

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Xing Wu Zhu, J S Friedland

Abstract

Multinucleate giant cells (MGC) are characteristic of tuberculous granulomas, but their function is not well understood. In a comparative study, we investigated regulation of chemokine secretion by MGC generated using 5 microg/ml ConA and 1000 IU/ml IFN-gamma. After 72-h differentiation of MGC cultures, CXCL8, CCL2 and CCL3 concentrations were 9540+/-110 pg/ml, 11190+/-2210 pg/ml and 19440+/-440 pg/ml respectively all significantly higher than in MDM (P<0.01). There was associated increased chemokine gene expression. M.tb stimulation of MGC, MDM and monocytes increased CXCL8 secretion. M.tb increased monocyte CCL2 secretion, whereas MGC and MDM secreted CCL2 constitutively. CXCL10 secretion was induced in M.tb-stimulated MDM and constitutive in MGC. All cell types responded to M.tb with CCL3 secretion. Monocyte chemokine secretion was associated with increased gene expression, whereas M.tb-stimulated MGC principally upregulated CCL3 gene expression. In summary, differentiating MGC express genes for and secrete chemokines which regulate cell influx to sites of infection. Established MGC will contribute to cell recruitment to granuloma, but this may not depend on exposure to the pathogen.

References

Feb 1, 1988·The Journal of Experimental Medicine·A McInnes, D M Rennick
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J B WeinbergM A Misukonis
Apr 1, 1997·American Journal of Respiratory and Critical Care Medicine·K KurashimaK Matsushima
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·W PetersJ D Ernst
Mar 16, 2002·Infection and Immunity·Jussi J SaukkonenHardy Kornfeld
Jul 8, 2003·The Journal of Pathology·Katsunori JinnouchiMotohiro Takeya
May 1, 2004·The Journal of Biological Chemistry·Melissa I WickremasingheJon S Friedland
Jun 26, 2004·Journal of Leukocyte Biology·Robert D Stout, Jill Suttles
Dec 8, 2004·The American Journal of Pathology·Themis R KyriakidesPaul Bornstein

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Citations

Jan 11, 2007·Immunology and Cell Biology·Bernadette M Saunders, Warwick J Britton
Mar 2, 2013·Genetic Testing and Molecular Biomarkers·Chen ShenA-Dong Shen
Mar 5, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Jae-Joon Yim, Paramasivam Selvaraj
Jul 25, 2012·Infection and Immunity·Abul K AzadLarry S Schlesinger
Aug 10, 2013·Drug Design, Development and Therapy·Wei-wei TianHong-sheng Wang
Jan 11, 2014·BMC Medical Genetics·Danielle CarpenterJohn A L Armour
Nov 22, 2012·Cytokine & Growth Factor Reviews·Samantha R Slight, Shabaana A Khader
Oct 8, 2008·The Journal of Infection·Patricia Méndez-Samperio
Sep 18, 2009·International Journal of Experimental Pathology·Brandon L PlattnerJesse M Hostetter
May 3, 2016·The Canadian Journal of Cardiology·Yuki ImaizumiKazuomi Kario
Nov 21, 2015·Scientific Reports·John D BlischakYoav Gilad
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·J Christian J RayDenise E Kirschner
Feb 16, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mohammad Fallahi-SichaniJennifer J Linderman
May 23, 2008·Mycopathologia·Magda Paula Pereira do NascimentoMaria Terezinha Serrão Peraçoli
May 30, 2016·Mycopathologia·Renato do Prado Gomes PedreiraJoão Adolfo Costa Hanemann

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