Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica

Scientific Reports
Olga Hernández de la CruzCesar Lopez-Camarillo

Abstract

Entamoeba histolytica is the intestinal parasite responsible for human amoebiasis that is a leading cause of death in developing countries. In this protozoan, heterogeneity in DNA content, polyploidy and genome plasticity have been associated to alterations in mechanisms controlling DNA replication and cell division. Studying the function of the transcription factor EhPC4, we unexpectedly found that it is functionally related to DNA replication, and multinucleation. Site-directed mutagenesis on the FRFPKG motif revealed that the K127 residue is required for efficient EhPC4 DNA-binding activity. Remarkably, overexpression of EhPC4 significantly increased cell proliferation, DNA replication and DNA content of trophozoites. A dramatically increase in cell size resulting in the formation of giant multinucleated trophozoites (polykaryon) was also found. Multinucleation event was associated to cytokinesis failure leading to abortion of ongoing cell division. Consistently, genome-wide profiling of EhPC4 overexpressing trophozoites revealed the up-regulation of genes involved in carbohydrates and nucleic acids metabolism, chromosome segregation and cytokinesis. Forced overexpression of one of these genes, EhNUDC (nuclear movement prote...Continue Reading

References

Oct 1, 1988·Experimental Parasitology·E OrozcoF Hernández
Sep 1, 1995·The Journal of Eukaryotic Microbiology·J A DvorakC W Hallahan
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·R Nickel, E Tannich
Nov 14, 1997·Nature Structural Biology·J BrandsenP Gros
Sep 2, 1998·The EMBO Journal·S WertenM Meisterernst
Feb 22, 2002·Cellular Microbiology·Suchismita Das, Anuradha Lohia
Apr 8, 2003·Journal of Cell Science·Jonathan P AumaisLi-Yuan Yu-Lee
Jun 5, 2003·Genes & Development·Olga Calvo, James L Manley
Jul 11, 2003·Developmental Cell·Tianhua ZhouRaymond L Erikson
Feb 18, 2004·Molecular and Cellular Biology·Sourav BanerjeeTapas K Kundu
Sep 7, 2004·The Journal of Biological Chemistry·Yumiko Saito-NakanoTomoyoshi Nozaki
Jun 16, 2005·Experimental Parasitology·E Azuara-LiceagaE Orozco
Feb 25, 2006·Molecular and Biochemical Parasitology·Ohad FisherSerge Ankri
May 24, 2006·Molecular and Biochemical Parasitology·Luis Enrique Arias-RomeroMiguel Vargas
Sep 20, 2006·Molecular and Cellular Biology·Chandrima DasTapas K Kundu
Jun 27, 2007·Bioscience Reports·Anuradha LohiaPromita Ghosh Dastidar
Mar 19, 2008·Infection and Immunity·Shubhra Majumder, Anuradha Lohia
Aug 21, 2008·PLoS Neglected Tropical Diseases·Chandrama MukherjeeAnuradha Lohia
Dec 3, 2008·The Journal of Cell Biology·Oliver MortusewiczHeinrich Leonhardt
Jan 1, 1997·European Psychiatry : the Journal of the Association of European Psychiatrists·S SaxenaUNKNOWN WHOQOL Group
Jun 30, 2010·PLoS Pathogens·Laura H OkagakiKirsten Nielsen
Nov 8, 2011·Molecular Cell·Timothy W SikorskiStephen Buratowski
Sep 14, 2012·Molecular Biology of the Cell·Alicia GarcíaOlga Calvo
Nov 20, 2013·Wiley Interdisciplinary Reviews. RNA·César López-CamarilloLaurence A Marchat

❮ Previous
Next ❯

Citations

Apr 19, 2017·The Journal of Biological Chemistry·Wezley C GriffinKevin D Raney
Apr 11, 2018·Scientific Reports·Juan David Ospina-VillaLaurence A Marchat
Dec 12, 2019·Frontiers in Cellular and Infection Microbiology·Tansy C Hammarton
Sep 29, 2017·The Journal of Microbiology·Juan David Ospina-VillaLaurence A Marchat
Jul 5, 2018·Frontiers in Cellular and Infection Microbiology·Jesús Valdés-FloresLaurence A Marchat
Sep 7, 2021·FEBS Open Bio·América Itzallana Salgado-MartínezLaurence A Marchat

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoretic mobility shift
confocal microscopy
transfection
immunoprecipitation
Assay
FACS

Software Mentioned

MEGA
WCIF ImageJ
Axiovision
CellQuest
BLAST
GenePix5
Leica
GraphPad Prism
ClustalW
VMD viewer

Related Concepts

Related Feeds

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.