Multiomics global landscape of stemness-related gene clusters in adipose-derived mesenchymal stem cells.

Stem Cell Research & Therapy
Guan-Ming LuHong-Mian Li

Abstract

Adipose-derived mesenchymal stem cells (AD-MSCs) are a type of stem cell that is abundant and widely used. The molecular characteristics of AD-MSCs from different passages from donors of different ages have not been well elucidated. Six kinds of AD-MSCs ((E1, E2, E3, Y1, Y2, and Y3) with E denoting cells derived from an elderly patient, Y denoting cells derived from a young patient, and 1, 2, and 3 representing passages 3, 6, and 10) were obtained from human abdominal adipose tissue. We obtained the protein expression profile, the mRNA expression profile, the lncRNA expression profile, and the methylation profile of each kind of AD-MSC by sequencing. After calculating the stemness indices, genes related to stemness were extracted. The multiomics correlation analysis was performed in the stemness-related genes. In addition, short time-series expression miner (STEM) analysis was performed for all cell passages and donor ages. To further explore the biological functions of the stemness-related genes, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Finally, the lncRNA-KEGG network and transcription factor (TF)-KEGG network were constructed based on the RNAInter database and TR...Continue Reading

References

Oct 11, 1996·Science·Y Li, R Benezra
Jul 9, 1997·Biochemical and Biophysical Research Communications·H TsurugaH Nojima
May 24, 2005·Journal of Medical Genetics·H CaldasR A Altura
Nov 4, 2005·Plastic and Reconstructive Surgery·Yun-Ying ShiMichael T Longaker
Apr 7, 2006·BMC Bioinformatics·Jason Ernst, Ziv Bar-Joseph
Aug 16, 2008·Aesthetic Plastic Surgery·V Cervelli, P Gentile
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Jul 10, 2009·Biometrical Journal. Biometrische Zeitschrift·James A Koziol, Zhenyu Jia
May 21, 2010·The Journal of Craniofacial Surgery·Pietro GentileValerio Cervelli
Jul 28, 2010·Molecular Biology of the Cell·Delquin Gong, James E Ferrell
Nov 11, 2010·Methods in Molecular Biology·Michael KohlBettina Warscheid
Mar 23, 2011·Advances in Skin & Wound Care·Valerio CervelliBarbara De Angelis
Oct 19, 2011·Surgical Innovation·Pietro GentileValerio Cervelli
Mar 21, 2012·Cancer Letters·Simone Fulda
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Jan 18, 2013·BMC Bioinformatics·Sonja HänzelmannJustin Guinney
Jul 10, 2013·International Wound Journal·Fabio NicoliValerio Cervelli
Jun 10, 2014·The Journal of Clinical Investigation·Emma L BapleAndrew H Crosby
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Jul 17, 2015·Plastic and Reconstructive Surgery. Global Open·Pietro GentileValerio Cervelli
Sep 20, 2015·Methods : a Companion to Methods in Enzymology·Stephen M RichardsonAli Mobasheri
Jun 1, 2016·The Enzymes·E M BoehmM T Washington
Oct 7, 2017·Science·Adel Al JordAlice Meunier

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
proteome sequencing

Software Mentioned

ClueGO
RNAInter
clusterProfiler
featureCounts
Cytoscape
TRRUST
STEM
limma
R
Hmisc

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.