Multiparameter flow cytometric analysis reveals low percentage of bone marrow hematogones in myelodysplastic syndromes

American Journal of Clinical Pathology
Sepideh Maftoun-BanankhahWeina Chen

Abstract

Diagnosis of myelodysplastic syndromes (MDS) could be difficult. We explored the usefulness of the enumeration of maturing B-lineage precursors (hematogones) by multiparameter flow cytometric analysis in the diagnosis of MDS in bone marrow (BM) specimens. We evaluated 111 MDS, 120 non-MDS (most with cytopenias; control group 1), and 41 noncytopenic lymphoma staging BM (control group 2) specimens. The percentage of total hematogones was significantly lower in MDS (median, 0%; mean, 0.10%) compared with non-MDS (control group 1, median, 0.38%, and mean, 0.91%; control group 2, median, 0.38%, and mean, 0.60%; P < .0001), as was the percentage of the most immature (stage I) hematogones. Thus, hematogone enumeration may serve as a biomarker to aid in the diagnosis of MDS. Interestingly, the percentage of hematogones was not significantly different between MDS subgroups or patients with MDS with and without chromosomal abnormalities, implying that a defect in maturing B-cell precursors may be an early event in the pathogenesis of MDS.

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Citations

Dec 17, 2009·Leukemia & Lymphoma·Deborah W SevillaBachir Alobeid
Dec 1, 2013·Surgical Pathology Clinics·Dita Gratzinger, Peter L Greenberg
Apr 24, 2012·American Journal of Clinical Pathology·Alexandra M HarringtonSteven H Kroft
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Jun 16, 2011·Cytometry. Part B, Clinical Cytometry·Matteo Giovanni Della PortaUNKNOWN Italian Society of Cytometry (GIC)
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Apr 19, 2016·International Journal of Molecular Sciences·Eugenia Flores-Figueroa, Dita Gratzinger
Mar 23, 2017·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Lisa Sutton, Nkechi Mba
Mar 12, 2019·American Journal of Clinical Pathology·Jason M SchenkelDavid M Dorfman

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