Multiparametric image analysis reveals role of Caveolin1 in endosomal progression rather than internalization of EGFR

FEBS Letters
Hannah Schmidt-GlenewinkelRoland Eils

Abstract

Endosomes constitute a central layer in the regulation of growth factor signaling. We applied flow cytometry, confocal microscopy and automated image quantification to define the role of Caveolin1 (Cav1) in epidermal growth factor (EGF) receptor (i) internalization and (ii) endosomal trafficking. Antisense-downregulation of Cav1 did not affect internalization of EGF:EGFR-complexes from the plasma membrane. Instead, Cav1-knockdown had a profound effect on endosomal trafficking and caused a shift in EGF vesicle distribution towards Rab7-negative compartments at late timepoints. Moreover, image quantification with single-endosome resolution revealed that EGF:Cav1-complexes undergo a maturation pattern reminiscent of late endosomes. Our data suggest a model in which Caveolin1 acts upon EGF endosomes internalized via the Clathrin-pathway and functions at the transition from early to late endosomes.

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Citations

Aug 25, 2015·Developmental Cell·Christina D Cota, Brad Davidson
Aug 11, 2016·Biophysical Journal·Jinhui LiYan Chen
Nov 8, 2016·Human Pathology·Jelena JankovićSvetlana Paskaš
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Aug 1, 2019·Biomolecules·America CamposAndrew Frederick Geoffery Quest
Jul 19, 2013·Cancer Research·Andrei TurtoiVincent Castronovo
May 18, 2021·Frontiers in Cell and Developmental Biology·Chia-Hsing ShenLi-Chung Hsu

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