Multiparametric Optical Bioimaging Reveals the Fate of Epoxy Crosslinked Biomeshes in the Mouse Subcutaneous Implantation Model

Frontiers in Bioengineering and Biotechnology
Vadim ElaginPeter S Timashev

Abstract

Biomeshes based on decellularized bovine pericardium (DBP) are widely used in reconstructive surgery due to their wide availability and the attractive biomechanical properties. However, their efficacy in clinical applications is often affected by the uncontrolled immunogenicity and proteolytic degradation. To address this issue, we present here in vivo multiparametric imaging analysis of epoxy crosslinked DBPs to reveal their fate after implantation. We first analyzed the structure of the crosslinked DBP using scanning electron microscopy and evaluated proteolytic stability and cytotoxicity. Next, using combination of fluorescence and hypoxia imaging, X-ray computed microtomography and histology techniques we studied the fate of DBPs after subcutaneous implantation in animals. Our approach revealed high resistance to biodegradation, gradual remodeling of a surrounding tissue forming the connective tissue capsule and calcification of crosslinked DBPs. These changes were concomitant to the development of hypoxia in the samples within 3 weeks after implantation and subsequent induction of angiogenesis and vascularization. Collectively, presented approach provides new insights on the transplantation of the epoxy crosslinked biomesh...Continue Reading

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Citations

Sep 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elvira R GafarovaPeter S Timashev
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Irina Yu ZhuravlevaBoris A Trofimov
May 8, 2021·Journal of Cell Science·Ruslan I DmitrievMargarida M Barroso
Aug 15, 2021·Journal of Biomedical Materials Research. Part a·Irina Yu ZhuravlevaNatalia R Nichay

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Methods Mentioned

BETA
fluorescence stereomicroscopy
X-ray
confocal microscopy
phosphorescence
confocal
fluorescence stereo microscopy
fluorescence imaging
light scattering

Software Mentioned

Statistica
GraphPad Prism
GraphPad
LAS
ImageJ

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