Multiphoton crosslinking for biocompatible 3D printing of type I collagen

Biofabrication
Alex BellVasile Nistor

Abstract

Multiphoton fabrication is a powerful technique for three-dimensional (3D) printing of structures at the microscale. Many polymers and proteins have been successfully structured and patterned using this method. Type I collagen comprises a large part of the extracellular matrix for most tissue types and is a widely used cellular scaffold material for tissue engineering. Current methods for creating collagen tissue scaffolds do not allow control of local geometry on a cellular scale. This means the environment experienced by cells may be made up of the native material but unrelated to native cellular-scale structure. In this study, we present a novel method to allow multiphoton crosslinking of type I collagen with flavin mononucleotide photosensitizer. The method detailed allows full 3D printing of crosslinked structures made from unmodified type I collagen and uses only demonstrated biocompatible materials. Resolution of 1 μm for both standing lines and high-aspect ratio gaps between structures is demonstrated and complex 3D structures are fabricated. This study demonstrates a means for 3D printing with one of the most widely used tissue scaffold materials. High-resolution, 3D control of the fabrication of collagen scaffolds wil...Continue Reading

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Citations

Apr 10, 2016·Advanced Healthcare Materials·Mark A Skylar-ScottMehmet Fatih Yanik
Apr 9, 2019·Advanced Materials·Anke SteierJoerg Lahann
Oct 8, 2016·Biofabrication·Yi-Cheun YehJason A Burdick
Apr 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hiroyuki Tetsuka, Su Ryon Shin
Aug 23, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Noam Zuela-Sopilniak, Jan Lammerding
Aug 16, 2019·Tissue Engineering and Regenerative Medicine·Zahra RashidbenamMin Hwei Ng
Dec 8, 2020·OSA Continuum·Behrang H HamadaniClarence Zarobila
Jan 21, 2021·Chemical Society Reviews·Jinhua Li, Martin Pumera
Mar 30, 2021·Progress in Polymer Science·Xuan MuDavid L Kaplan

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