Multiphoton excitation fluorescence microscopy of 5-aminolevulinic acid induced fluorescence in experimental gliomas

Lasers in Surgery and Medicine
Sven Rainer KantelhardtAlf Giese

Abstract

The clinical usefulness of 5-ALA guided detection of tumor tissue has been demonstrated for a number of malignancies. However, current techniques of intraoperative detection of protoporphyrin IX fluorescence in situ do not offer subcellular resolution. Therefore, discrimination of non-specific 5-ALA induced fluorescence remains difficult. In this study we have used an orthotopic glioma model to analyze PpIX fluorescence in tumor tissue and normal brain by multiphoton excitation microscopy after intraperitoneal administration of 5-ALA. A DermaInspect in vivo imaging system was used for autofluorescence measurements at 750 nm excitation and detection in the green channel of a standard photomultiplier module. For detection of PpIX fluorescence at different excitation wavelengths a red sensitive version of the photomultiplier and a filter combination of short pass filters and a color glass long pass filter was used restricting the sensitivity in the red channel to a range of 580-700 nm. Multiphoton microscopy allowed a higher structural definition of tumor tissue based on the excitation of 5-ALA induced PpIX fluorescence compared to autofluorescence imaging. The high resolution of multiphoton microscopy allowed discrimination of fl...Continue Reading

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Citations

Mar 20, 2010·Stem Cell Reviews and Reports·Shengwen Calvin LiWilliam G Loudon
Apr 2, 2010·Chemical Reviews·Mikhail Y Berezin, Samuel Achilefu
Jul 9, 2010·PloS One·Sven R KantelhardtDonna J Arndt-Jovin
Feb 25, 2009·Acta neurochirurgica·Sven Rainer KantelhardtAlf Giese
Jun 11, 2010·Acta neurochirurgica·Daniel ColucciaHans Landolt
Oct 13, 2012·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Michael J ColditzRosalind L Jeffree
Jul 31, 2010·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Brian W PogueKeith D Paulsen
Mar 24, 2009·IEEE Transactions on Nanobioscience·Donna J Arndt-JovinThomas M Jovin Ast
Oct 13, 2009·Trends in Cell Biology·Paolo P ProvenzanoPatricia J Keely
Nov 1, 2013·Photonics & Lasers in Medicine·Kishore R RollakantiEdward V Maytin
Jun 2, 2020·Frontiers in Physiology·Zhaojun NieQiyin Fang
Feb 3, 2016·Journal of Neuro-oncology·Sven R KantelhardtAlf Giese
Aug 17, 2021·Frontiers in Oncology·Barbara KieselGeorg Widhalm

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