Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin

Biophysical Journal
Barry R MastersEnrico Gratton

Abstract

Multiphoton excitation microscopy at 730 nm and 960 nm was used to image in vivo human skin autofluorescence from the surface to a depth of approximately 200 microm. The emission spectra and fluorescence lifetime images were obtained at selected locations near the surface (0-50 microm) and at deeper depths (100-150 microm) for both excitation wavelengths. Cell borders and cell nuclei were the prominent structures observed. The spectroscopic data suggest that reduced pyridine nucleotides, NAD(P)H, are the primary source of the skin autofluorescence at 730 nm excitation. With 960 nm excitation, a two-photon fluorescence emission at 520 nm indicates the presence of a variable, position-dependent intensity component of flavoprotein. A second fluorescence emission component, which starts at 425 nm, is observed with 960-nm excitation. Such fluorescence emission at wavelengths less than half the excitation wavelength suggests an excitation process involving three or more photons. This conjecture is further confirmed by the observation of the super-quadratic dependence of the fluorescence intensity on the excitation power. Further work is required to spectroscopically identify these emitting species. This study demonstrates the use of ...Continue Reading

Citations

Apr 17, 2010·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·M Kaatz, K König
May 5, 2006·Histochemistry and Cell Biology·Bao-Gui WangKarl-Juergen Halbhuber
Jan 26, 2012·Annals of Biomedical Engineering·Laura Marcu
Feb 16, 2005·Journal of Fluorescence·Christof BuehlerPeter T C So
Apr 10, 2003·The Annals of Thoracic Surgery·Hemant S ThatteShukri F Khuri
Aug 2, 2002·European Journal of Pharmacology·Fritjof Helmchen, Jack Waters
Jun 17, 1999·Biophysical Chemistry·I GryczynskiJ R Lakowicz
Nov 8, 2001·Experimental Hematology·J R Allport, R Weissleder
Feb 1, 2003·Experimental Gerontology·G J Schütz, P Hinterdorfer
Aug 12, 2000·Free Radical Biology & Medicine·T ParasassiF Ursini
Aug 7, 1999·Progress in Retinal and Eye Research·M Böhnke, B R Masters
Oct 11, 2011·Tissue Engineering. Part C, Methods·Roman DittmarKeita Ito
Jun 29, 2004·Plastic and Reconstructive Surgery·Fernando A NavarroDennis P Orgill
Mar 16, 2012·Cardiology in Review·Kimberly DaoGeorge Hines
Nov 9, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Thomas AbrahamBruce McManus
Jan 24, 2009·Photochemistry and Photobiology·Kerry M Hanson, Christopher J Bardeen
Jan 28, 2009·Photochemistry and Photobiology·Tong YeWarren S Warren
Jul 26, 2003·Journal of Biomedical Optics·Chen-Yuan DongPeter So
Dec 1, 2004·Journal of Biomedical Optics·Barry R MastersEnrico Gratton
Sep 24, 2005·Journal of Biomedical Optics·Edward E GravesVasilis Ntziachristos
May 5, 2007·Journal of Biomedical Optics·Hsin-Yuan TanChen-Yuan Dong
Jul 8, 2008·Journal of Biomedical Optics·Tsung-Jen WangFung-Rong Hu
Nov 22, 2008·Journal of Biomedical Optics·Jonathan A PaleroHans C Gerritsen
Nov 22, 2008·Journal of Biomedical Optics·Aisada Uchugonova, Karsten König
May 23, 2012·Journal of Biomedical Optics·Da-Kang YaoLihong V Wang
Dec 4, 2010·Science·Brian G SaarX Sunney Xie
Nov 10, 2001·Annual Review of Biomedical Engineering·P T SoK M Berland
Oct 11, 2005·Annual Review of Cell and Developmental Biology·John CondeelisJeffrey E Segall
Mar 18, 2006·Nephron. Experimental Nephrology·Kenneth W Dunn, Pamela A Young
Apr 27, 2001·Radiology·R Weissleder, U Mahmood

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.