PMID: 9188272Jun 1, 1997Paper

Multiphoton-excited visible emission by serotonin solutions

Photochemistry and Photobiology
J B ShearWatt W Webb

Abstract

Nonlinear excitation of the neurotransmitter serotonin (5HT) in aqueous solution is shown to generate a blue-green-emitting photoproduct in addition to UV fluorescence characteristic of native 5HT. The visible emission rate in diffusional steady-state measurements scales as the sixth power of excitation intensity, demonstrating that absorption of six near-IR photons is required to generate emission of one visible photon. Transient measurements reveal that this process is composed of two sequential nonlinear steps, the first excited by four photons and the second by two photons. These results, in combination with measurements of multiphoton-excited serotonin UV fluorescence, support a model in which 5HT is photochemically transformed as a consequence of four-photon absorption (Etot approximately 6 eV) to a photoproduct that then emits in the visible region via two-photon excitation. A minimum bound of approximately 10(-51) cm4 s photon-1 is observed for the two-photon emission action cross section at 830 nm. Photoionization, rather than reaction with a dissolved oxygen species, appears to be the primary mechanism for generation of the blue-green-emitting photoproduct. The peak intensities required to generate significant blue-gr...Continue Reading

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Citations

Aug 13, 1998·Journal of Neuroscience Methods·V ParpuraP G Haydon
Oct 31, 2009·Analytical Chemistry·Eric T RitschdorffJason B Shear
Feb 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·L SacconiW W Webb
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Matthew L Plenert, Jason B Shear
Jan 24, 2009·Photochemistry and Photobiology·Roger H BisbyAnthony W Parker
Jul 26, 2003·Journal of Biomedical Optics·Mary E DickinsonScott E Fraser
Nov 10, 2001·Annual Review of Biomedical Engineering·P T SoK M Berland
Mar 12, 2004·Annual Review of Physical Chemistry·X S Xie, J K Trautman
Oct 24, 2012·Case Reports in Gastrointestinal Medicine·Michael A SpinnerC William Wester
Jun 8, 2007·Archives of Biochemistry and Biophysics·Stanley W BotchwayRoger H Bisby
Feb 3, 2004·Lasers in Surgery and Medicine·Francesco CrespiGiovanni Bottiroli
Jan 6, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Roger H BisbyAnthony W Parker
Mar 25, 2020·Biomedical Optics Express·Guillermo A Hernández-MendozaFrancisco F De-Miguel
Oct 25, 2001·Journal of the American Chemical Society·M J GordonJ B Shear
Mar 26, 2014·ACS Chemical Neuroscience·Bidyut SarkarSudipta Maiti
Jul 18, 2001·Journal of the American Chemical Society·M J Gordon, J B Shear

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