Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds

Biomaterials Research
Min Jeong KimDong-Wook Han

Abstract

Hydrogels can serve as three-dimensional (3D) scaffolds for cell culture and be readily injected into the body. Recent advances in the image technology for 3D scaffolds like hydrogels have attracted considerable attention to overcome the drawbacks of ordinary imaging technologies such as optical and fluorescence microscopy. Multiphoton microscopy (MPM) is an effective method based on the excitation of two-photons. In the present study, C2C12 myoblasts differentiated in 3D gelatin hydroxyphenylpropionic acid (GHPA) hydrogels were imaged by using a custom-built multiphoton excitation fluorescence microscopy to compare the difference in the imaging capacity between conventional microscopy and MPM. The physicochemical properties of GHPA hydrogels were characterized by using scanning electron microscopy and Fourier-transform infrared spectroscopy. In addition, the cell viability and proliferation of C2C12 myoblasts cultured in the GHPA hydrogels were analyzed by using Live/Dead Cell and CCK-8 assays, respectively. It was found that C2C12 cells were well grown and normally proliferated in the hydrogels. Furthermore, the hydrogels were shown to be suitable to facilitate the myogenic differentiation of C2C12 cells incubated in differen...Continue Reading

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Citations

Jun 29, 2017·Journal of Biomaterials Science. Polymer Edition·Yong Cheol ShinDong-Wook Han
Aug 28, 2019·Pharmaceutics·Anca OnaciuIoana Berindan-Neagoe
Aug 30, 2020·Bioengineering·Moon Sung KangDong-Wook Han
Jan 21, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jeon Il Kang, Kyung Min Park
Jun 3, 2021·International Journal of Molecular Sciences·Mohan VedhanayagamDong-Wook Han

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Methods Mentioned

BETA
optical
confocal microscopy
scanning electron microscopy
fluorescence microscopy
ELISA

Software Mentioned

ImageJ
MPM

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