Multiphoton Intravital Microscopy of Mandibular Draining Lymph Nodes: A Mouse Model to Study Corneal Immune Responses.

Frontiers in Immunology
Maria J LopezPedram Hamrah

Abstract

Multiphoton intravital microscopy (MP-IVM) is a powerful tool to image cells in vivo. Its application in immunology research has opened new horizons, allowing intravital imaging of leukocytes at the single-cell level. A transparent cornea is vital to retain vision. As an immune privileged site, a rapid innate response to foreign antigens is crucial in clearing opportunistic bacterial and viral pathogens, and minimizing collateral structural damage to the cornea. Furthermore, dissecting the mechanisms and preventing the immunological rejection process after corneal transplantation is imperative to retain sight. Therefore, understanding the underlying mechanisms behind corneal immunity, specifically the process of antigen presentation and adaptive immunity in the mandibular draining lymph nodes (dLNs) in vivo, is crucial. Attempts of intravital imaging of mandibular dLNs have yielded little success to date, due to breathing artifacts and the location that is difficult to access. Herein, we present the first MP-IVM mouse model of the mandibular dLNs, utilizing transgenic mice in which CD11c+ cells are fluorescently labeled. Furthermore, we demonstrate that CD11c-YFP+ cells are localized mainly in the parafollicular cortex (T cell ...Continue Reading

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Citations

Aug 17, 2020·Investigative Ophthalmology & Visual Science·Paul G McMenaminSarah E Coupland
Mar 3, 2021·ACS Nano·Carlos Sanchez-CanoWolfgang J Parak

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Methods Mentioned

BETA
FACS
dissection
transgenic
flow cytometry
confocal microscopy

Software Mentioned

Prism Graphpad
Imaris
FlowJo
Prairie View
MP
IVM

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