Multiphoton microscopy: A novel diagnostic method for solid tumors in a prospective pediatric oncologic cohort, an experimental study

International Journal of Surgery
Oliver J MuenstererJan Gödeke

Abstract

The prognosis of solid pediatric tumors strongly correlates with accurate staging and complete local control. Currently, surgeons rely on macroscopic cues and intraoperative cryosection to determine resection borders. Multiphoton Microscopy (MPM) is a real time technique that allows imaging of tissue without time-consuming tissue processing. This pilot study evaluates the diagnostic potential of MPM in pediatric solid tumors compared to routine histopathology. Slides of pediatric tumor samples (nephroblastoma and neuroblastoma [n = 2]; ganglioneuroma, pleuropulmonary blastoma, hepatocellular carcinoma [n = 1]) were prepared to allow direct comparison of MPM with conventional light microscopy. Additionally, we applied MPM to native tumor tissue blocks to evaluate direct visualization of malignant cells through the tumor capsule. Images were interpreted by an attending surgical pathologist. Detectability of tumor-specific features was compared between MPM and conventional histology. A total of 7 tumors from 7 recruited patients were analyzed. All MPM images were accurate in diagnosing typical criteria of each particular neoplasm. In addition, MPM clearly visualized tumors through the capsule without sectioning or labeling procedu...Continue Reading

Citations

Oct 28, 2019·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·Jan GödekeS Rohleder
Aug 4, 2018·Frontiers in Aging Neuroscience·Carmen Hierro-BujalanceMonica Garcia-Alloza

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