Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe.

Journal of Biophotonics
Gangjun LiuZhongping Chen

Abstract

We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

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Citations

Mar 20, 2010·Stem Cell Reviews and Reports·Shengwen Calvin LiWilliam G Loudon
Apr 5, 2011·Journal of Biomedical Optics·Gangjun Liu, Zhongping Chen
Oct 7, 2016·Chemical Society Reviews·Yizhong ShenHong-Yuan Chen
Jan 15, 2014·Nature Photonics·C Xu, F W Wise
May 26, 2016·Journal of Biomedical Optics·Tyler H TateJennifer Kehlet Barton
Apr 11, 2018·Journal of Biomedical Optics·Benjamin CromeyBhaskar Banerjee
Dec 24, 2018·Journal of Biomedical Optics·Lin HuangShuo Tang
Jul 19, 2012·Biomedical Optics Express·Bai NieMarcos Dantus
Feb 15, 2012·Journal of Biophotonics·Vladimir A HovhannisyanChen-Yuan Dong
Aug 27, 2014·Journal of Biomedical Optics·Sarah Saint-JalmHervé Rigneault
May 15, 2013·Biomedical Optics Express·David M HulandChris Xu
Sep 30, 2015·Biomedical Optics Express·Erin S Lamb, Frank W Wise

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