Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: application to microcrystalline and membrane protein preparations

Journal of Magnetic Resonance
T Gopinath, Gianluigi Veglia

Abstract

Solid-state NMR spectroscopy of proteins is a notoriously low-throughput technique. Relatively low-sensitivity and poor resolution of protein samples require long acquisition times for multidimensional NMR experiments. To speed up data acquisition, we developed a family of experiments called Polarization Optimized Experiments (POE), in which we utilized the orphan spin operators that are discarded in classical multidimensional NMR experiments, recovering them to allow simultaneous acquisition of multiple 2D and 3D experiments, all while using conventional probes with spectrometers equipped with one receiver. POE allow the concatenation of multiple 2D or 3D pulse sequences into a single experiment, thus potentially combining all of the aforementioned advances, boosting the capability of ssNMR spectrometers at least two-fold without the addition of any hardware. In this perspective, we describe the first generation of POE, such as dual acquisition MAS (or DUMAS) methods, and then illustrate the evolution of these experiments into MEIOSIS, a method that enables the simultaneous acquisition of multiple 2D and 3D spectra. Using these new pulse schemes for the solid-state NMR investigation of biopolymers makes it possible to obtain s...Continue Reading

References

Nov 1, 1985·Physical Review. B, Condensed Matter·D Suter, R R Ernst
May 2, 2001·Journal of Magnetic Resonance·R VerelB H Meier
Jan 10, 2002·Biochemistry·Alessandro MascioniGianluigi Veglia
Jul 16, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Robert Tycko
Sep 16, 2004·Journal of the American Chemical Society·Nicolas GiraudLyndon Emsley
Sep 29, 2004·Journal of Biomolecular NMR·D RovnyakG Wagner
Aug 10, 2007·Journal of Biomolecular NMR·W Trent FranksChad M Rienstra
Dec 18, 2007·Biochemistry·Nathaniel J TraasethGianluigi Veglia
Feb 13, 2008·The Journal of Chemical Physics·Thorsten MalyRobert G Griffin
Jul 19, 2008·Solid State Nuclear Magnetic Resonance·Masashi Fukuchi, K Takegoshi
Feb 28, 2009·Annual Review of Biophysics·Ann McDermott
Dec 6, 2011·Annual Review of Physical Chemistry·Mei HongFanghao Hu
Mar 1, 2012·Journal of Biomolecular NMR·Anders B NielsenBeat H Meier
Jul 18, 2012·Journal of Biomolecular NMR·Eriks Kupče, Lewis E Kay
Mar 23, 2013·Accounts of Chemical Research·Aaron J RossiniLyndon Emsley
May 17, 2013·The Journal of Chemical Physics·T Gopinath, Gianluigi Veglia
Sep 17, 2013·Current Opinion in Structural Biology·Yimin Miao, Timothy A Cross
Jan 15, 2014·Journal of Magnetic Resonance·Timothy A CrossAnna Wright
Apr 18, 2014·Biophysical Journal·Dylan T MurrayTimothy A Cross
May 29, 2014·Current Opinion in Structural Biology·Lindsay A Baker, Marc Baldus
Jun 20, 2014·Journal of Biomolecular NMR·Christoph WiedemannRamadurai Ramachandran

❮ Previous
Next ❯

Citations

Feb 10, 2016·Molecular Membrane Biology·Simon G Patching
Sep 24, 2016·Quarterly Reviews of Biophysics·Mohammed KaplanMarc Baldus
Nov 16, 2018·Chemical Communications : Chem Comm·Kumar Motiram-CorralTeodor Parella
Jan 25, 2019·Magnetic Resonance in Chemistry : MRC·Pau Nolis, Teodor Parella
Jul 1, 2017·Angewandte Chemie·Ēriks Kupče, Tim D W Claridge
May 4, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ivan V SergeyevAnn E McDermott
May 26, 2017·The Journal of Physical Chemistry. B·Rongchun ZhangAyyalusamy Ramamoorthy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.