Multiple actions of extracellular ATP and adenosine on calcium currents mediated by various purinoceptors in neurons of nucleus tractus solitarius

Neuroscience Research
Yoshihito AokiTakashi Suzuki

Abstract

Whole-cell patch-clamp recordings were performed on freshly dissociated nucleus tractus solitarius (NTS) of rat to determine the action of extracellular adenosine 5'-triphosphate (ATP) and adenosine (ADO) on voltage-dependent calcium channel (VDCC) currents (I(Ca)). Application of ATP and ATP-analog inhibited I(Ca). The rank order of potency of inhibition of I(Ca) was 2-methylthioATP (2-MeSATP) > ATP > adenosine 5'-diphosphate (ADP) > alpha,beta-methylene ATP (alpha,beta-MeATP) = uridine 5'-triphosphate (UTP). Application of ADO receptor agonists also inhibited I(Ca). The rank order of potency of inhibition of I(Ca) was N(6)-cyclohexyladenosine (CHA) > ADO > 2-(4-(2-carboxyethyl)phenylethylamino)adenosine-5'-N-ethylcarboxamideadenosine (CGS-21680) > N(6)-2-(4-aminophenyl)ethyladenosine (APNEA). Application of prepulse attenuated these inhibition. Both intracellular dialysis of guanosin 5'-O-(2-thiodiphosphate) (GDP-beta-S) and anti-G(i) antibody also attenuated these inhibition. L-, N- and P/Q-type VDCCs were inhibited by ATP. In contrast, N- and P/Q-type VDCCs were inhibited by ADO. In addition to inhibition, application of 100 microM ATP facilitated I(Ca). Intracellular dialysis of GDP-beta-S did not attenuate these facilitat...Continue Reading

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Citations

Feb 15, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Laszlo KölesPeter Illes
Jun 6, 2006·Respiratory Physiology & Neurobiology·Ying Cao, Gang Song
Jul 28, 2005·Journal of Neuroscience Methods·Mark DallasJim Deuchars
Aug 21, 2012·Respiratory Physiology & Neurobiology·Lin ZhangShu Yan Yu
Mar 10, 2017·American Journal of Physiology. Cell Physiology·Tim D OstrowskiDavid D Kline
Apr 18, 2013·Pharmacological Reviews·Detlev Boison

Related Concepts

Adenocard
Adenosine Triphosphate, Chromium Ammonium Salt
Dose-Response Relationship, Drug
Neurons
Methyladenine Receptors
Rats, Wistar
Nucleus Solitarius
Calcium Sparks
Interstitial Fluid
Purinergic Agonists

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