PMID: 9526065Apr 4, 1998Paper

Multiple actions of nitric oxide on voltage-dependent Ca2+ channels in mouse cerebral cortical neurons

Brain Research. Molecular Brain Research
S OhkumaK Kuriyama

Abstract

We investigated the effects of nitric oxide (NO) on voltage-dependent Ca2+ channels (VDCCs) by examining [45Ca2+]influx into mouse cerebral cortical neurons. S-nitroso-N-acetylpenicillamine (SNAP) induced a dose-dependent increase in [45Ca2+]influx, which was completely abolished by hemoglobin, tetrodotoxin and dibucaine. The NO-induced [45Ca2+influx was significantly inhibited by verapamil and omega-agatoxin VIA (omega-AGX), whereas omega-conotoxin GVIA (omega-CTX) had no effects on the NO-induced [45Ca2+]influx. KCl (30 mM) stimulated [45Ca2+]influx, and verapamil, omega-CTX and omega-AGX reduced the KCl-induced [45Ca2+]influx by about 40, 26 and 34%, respectively, indicating that the neurons used here possess L-, N- and P-typed VDCCs. SNAP itself reduced KCl-induced [45Ca2+]influx by about 28.5%. In the presence of both KCl and SNAP, omega-CTX showed no effects on the influx, while verapamil and omega-AGX significantly inhibited the influx and the concomitant presence of verapamil and omega-AGX completely abolished the influx. These results indicate that NO induces [45Ca2+] influx via the opening of L- and P-typed VDCCs subsequent to neuronal membrane depolarization and that NO itself inhibited the function of N-typed VDCC i...Continue Reading

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Feb 1, 1996·Brain Research. Molecular Brain Research·S OhkumaK Kuriyama

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Citations

May 30, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S ArnholdK Addicks
Feb 24, 2001·Journal of Neurochemistry·S OhkumaT Ohgi
Apr 27, 2002·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A De LaetJ-P Timmermans
Apr 25, 2006·Free Radical Biology & Medicine·Olivier LamotteDavid Wendehenne
Dec 21, 2006·Neuroreport·Agata Adamczyk, Joanna B Strosznajder
Apr 12, 2000·Journal of Neurophysiology·I M ElayanC R Auker

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