Abstract
We prepared multiple antigen constructs (MACs) using circumsporozoite (CS) protein-based B-epitopes from Plasmodium berghei, (PPPPNPND)2 and Plasmodium yoelii, (QGPGAP)3QG, along with a P. berghei T-helper epitope KQIRDSITEEWS. Mice were immunized with individual MACs in oil-in-water or water-in-oil vehicles containing block copolymer (P1005) and detoxified RaLPS (RaLPS) as well as other adjuvants. Sporozoite challenge results demonstrated that MACs in adjuvant could induce antibodies capable of active and passive protection. Water-in-oil vaccines induced the highest level of protection in mice immunized with either P. berghei or P. yoelii MACs. In a study aimed at co-eliciting immunity against P. berghei and P. yoelii, three immunizations with MACs induced protective antibodies against P. berghei but not P. yoelii parasite challenge. Therefore, it can be concluded that individually MACs are capable of inducing strong and protective immune responses to either species of rodent malaria, and that protection can be passively transferred. When MAC formulations were used together as a combined vaccine, P. berghei MACs induced a strong protective antibody response while P. yoelii MACs induced a weaker nonprotective response.
References
Jan 1, 1990·The Journal of Experimental Medicine·J P TamF Zavala
Feb 8, 1991·Science·Y CharoenvitS L Hoffman
Jan 1, 1991·Medical and Veterinary Entomology·R A WirtzR G Andre
Nov 1, 1987·The Journal of Experimental Medicine·F ZavalaV Nussenzweig
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A A LalT F McCutchan
Apr 24, 1987·Science·J E EganI Schneider
Jun 1, 1996·Vaccine·R C ReedA A Lal
Citations
Nov 13, 2001·Molecular and Biochemical Parasitology·A C UhlemannJ F Kun
Jun 8, 2001·Vaccine·J P LangeveldW D Hamilton
May 30, 2002·Vaccine·Benjamin L NicholasD Wakelin
Jul 31, 1998·Vaccine·V UdhayakumarA A Lal
Sep 29, 1999·Vaccine·C A MorenoE Nardin
Dec 13, 2006·Infection and Immunity·Anjali YadavaChristian F Ockenhouse
May 27, 2010·PloS One·D Lys GuilbridePatrick D L Guilbride
Nov 13, 2007·Microbial Pathogenesis·Gowrisankar RajamSandra Romero-Steiner
Dec 6, 2002·The Journal of General Virology·Shao-Hung WangShiau-Ting Hu
Nov 28, 2006·Malaria Journal·Peter LacknerKristian Pfaller
Dec 14, 2011·International Journal of Microbiology·Gowrisankar RajamEdwin W Ades
Sep 5, 2008·PloS One·Yara C BlancoFabio T M Costa
Nov 10, 2010·PloS One·Shigeto YoshidaHiroyuki Matsuoka
Oct 23, 2012·Parasitology International·Upma Bagai, Anil Pawar
Jan 1, 1999·Alternatives to Laboratory Animals : ATLA·P P LeenaarsD E Stewart-Tull
Jan 9, 1999·Journal of Virology·F R BrennanW D Hamilton
Oct 13, 2009·Vaccine·Dev ChandranVilluppanoor Alwar Srinivasan