Multiple aster formation is frequently observed in bovine oocytes retrieved from 1-day stored ovaries

Zygote : the Biology of Gametes and Early Embryos
H HaraS Hochi

Abstract

We have recently reported that multiple aster formation after in vitro fertilization (IVF) was one of the factors that negatively affected the developmental competence of vitrified-warmed bovine matured oocytes, and that short-term culture of the post-warm oocytes with an inhibitor of Rho-associated coiled-coil kinase (ROCK) suppressed the multiple aster formation and improved the blastocyst yield. The present study was conducted to investigate whether increased multiple aster formation following IVF was involved in impaired developmental competence of stored ovary-derived bovine oocytes. Oocytes retrieved from 1-day stored ovaries had lower developmental potential to day 8 blastocysts when compared with those from fresh ovaries (37 versus 63%). Immunostaining of α-tubulin 10 h post-IVF revealed that a higher incidence of multiple aster formation occurred in oocytes retrieved from stored ovaries than from fresh ovaries (31 versus 15%). Treatment of post-in vitro maturated (post-IVM) oocytes with ROCK inhibitor for 2 h significantly suppressed the incidence of multiple aster formation (10 versus 32% in the control group). However, the suppression effect of ROCK inhibitor on multiple aster formation in IVM/IVF oocytes did not imp...Continue Reading

References

Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Kirsi Riento, Anne J Ridley
Jan 22, 2004·Molecular Reproduction and Development·Yukihiro TeradaGerald Schatten
Oct 10, 2009·The Journal of Reproduction and Development·Nucharin SripunyaRangsun Parnpai
May 7, 2010·Zygote : the Biology of Gametes and Early Embryos·Yoshikazu NagaoYoku Kato
Feb 18, 2011·The Journal of Reproduction and Development·Hiromasa HaraShinichi Hochi
Jun 21, 2013·Biology of Reproduction·In-Sul HwangShinichi Hochi

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Citations

Apr 22, 2019·Reproduction in Domestic Animals = Zuchthygiene·Ruth Rodríguez-BermúdezMarta López-Alonso
Oct 22, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David J SchwartzmanAnil K Seth

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