Multiple Benefits of Plasmid-Mediated Quinolone Resistance Determinants in Klebsiella pneumoniae ST11 High-Risk Clone and Recently Emerging ST307 Clone

Frontiers in Microbiology
Judit DomokosDóra Szabó

Abstract

International high-risk clones of Klebsiella pneumoniae are among the most common nosocomial pathogens. Increased diversity of plasmid-encoded antimicrobial resistance genes facilitates spread of these clones causing significant therapeutic difficulties. The purpose of our study was to investigate fluoroquinolone resistance in extended-spectrum beta-lactamase (ESBL)-producing strains, including four K. pneumoniae and a single K. oxytoca, isolated from blood cultures in Hungary. Whole-genome sequencing and molecular typing including multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed in selected strains. Gene expression of plasmid-mediated quinolone resistance determinants (PMQR) was investigated by quantitative-PCR. MLST revealed that three K. pneumoniae strains belonged to ST11 and one to ST307 whereas K. oxytoca belonged to ST52. The isolates harbored different β-lactamase genes, however, all K. pneumoniae uniformly carried blaCTX-M-15. The K. pneumoniae isolates exhibited resistance to fluoroquinolones and carried various PMQR genes namely, two ST11 strains harbored qnrB4, the ST307 strain harbored qnrB1 and all K. pneumoniae harbored oqxAB efflux pump. Levofloxacin and moxifloxacin M...Continue Reading

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Citations

Mar 25, 2020·Microorganisms·José E VillacísMónica C Gestal
Jan 3, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Isabel CarvalhoPatrícia Poeta
Apr 6, 2021·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Jung Ok KimYeon-Joon Park

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Methods Mentioned

BETA
Electrophoresis
PCR

Software Mentioned

MLST
BacWGST
Primer Express
Trimmomatic
Fingerprinting II Informatix
PlasmidFinder
ResFinder
SPAdes Genome Assembler

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