PMID: 9555876Apr 29, 1998Paper

Multiple beta-ketothiolases mediate poly(beta-hydroxyalkanoate) copolymer synthesis in Ralstonia eutropha

Journal of Bacteriology
S SlaterK J Gruys

Abstract

Polyhydroxyalkanoates (PHAs) are a class of carbon and energy storage polymers produced by numerous bacteria in response to environmental limitation. The type of polymer produced depends on the carbon sources available, the flexibility of the organism's intermediary metabolism, and the substrate specificity of the PHA biosynthetic enzymes. Ralstonia eutropha produces both the homopolymer poly-beta-hydroxybutyrate (PHB) and, when provided with the appropriate substrate, the copolymer poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) (PHBV). A required step in production of the hydroxyvalerate moiety of PHBV is the condensation of acetyl coenzyme A (acetyl-CoA) and propionyl-CoA to form beta-ketovaleryl-CoA. This activity has generally been attributed to the beta-ketothiolase encoded by R. eutropha phbA. However, we have determined that PhbA does not significantly contribute to catalyzing this condensation reaction. Here we report the cloning and genetic analysis of bktB, which encodes a beta-ketothiolase from R. eutropha that is capable of forming beta-ketovaleryl-CoA. Genetic analyses determined that BktB is the primary condensation enzyme leading to production of beta-hydroxyvalerate derived from propionyl-CoA. We also report...Continue Reading

References

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Citations

May 27, 2008·Applied and Environmental Microbiology·Matthias RabergAlexander Steinbüchel
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hsien-Chung Tseng, Kristala L J Prather
Aug 2, 2014·Applied Microbiology and Biotechnology·Jingnan LuAnthony J Sinskey
Aug 19, 2015·Bioprocess and Biosystems Engineering·Jong-Min JeonYung-Hun Yang
Sep 4, 2015·Critical Reviews in Biotechnology·Elena VolodinaAlexander Steinbüchel
Sep 30, 2016·Microorganisms·Peter James StrongSteven Pratt
Feb 11, 2020·Frontiers in Bioengineering and Biotechnology·Young-Kyoung ParkJean-Marc Nicaud
Mar 27, 2002·Journal of Bacteriology·Sergio EncarnaciónJaime Mora
Sep 12, 2006·Nature Biotechnology·Anne PohlmannBotho Bowien
Aug 17, 2010·Journal of Bacteriology·Christopher J BrighamAnthony J Sinskey
Jun 27, 2013·World Journal of Microbiology & Biotechnology·Stanislav ObrucaIvana Marova
Mar 8, 2014·Applied Microbiology and Biotechnology·Estelle GrousseauAnthony J Sinskey
Feb 21, 2016·Applied and Environmental Microbiology·Kajan SriranganC Perry Chou
Apr 30, 2016·Bioprocess and Biosystems Engineering·María García-TorreiroJuan M Lema
Sep 3, 2004·Molecular Microbiology·Darby G Brown, Caitilyn Allen
May 17, 2012·Applied Microbiology and Biotechnology·Jingnan LuAnthony J Sinskey
Mar 7, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Eun-Jung KimKyung-Jin Kim
Nov 13, 2014·Frontiers in Bioengineering and Biotechnology·Esteban Martínez-GarcíaPablo I Nikel

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