PMID: 7545281May 1, 1995Paper

Multiple calcium channel subtypes in isolated rat chromaffin cells

Pflügers Archiv : European journal of physiology
Luis GandíaA G García

Abstract

By using the whole-cell configuration of the patch-clamp technique we have investigated the pharmacological properties of Ca2+ channels in short-term cultured rat chromaffin cells. In cells held at a membrane potential of --80 mV, using 10 mM Ba2+ as the charge carrier, only high-voltage-activated (HVA) Ca2+ channels were found. Ba2+ currents (IBa) showed variable sensitivity to dihydropyridine (DHP) Ca2+ channel agonists and antagonists. Furnidipine, a novel DHP antagonist, reversibly blocked the current amplitude by 22% and 48%, at 1 microM and 10 microM respectively, during short (15-50 ms) depolarizing pulses to 0 mV. The L-type Ca2+ channel agonist Bay K 8644 (1 microM) caused a variable potentiation of HVA currents that could be better appreciated at low rather than at high depolarizing steps. Increase of IBa was accompanied by a 20-mV shift in the activation curves for Ca2+ channels towards more hyperpolarizing potentials. Application of the conus toxin omega-conotoxin GVIA (GVIA; 1 microM) blocked 31% of IBa; blockade was irreversible upon removal of the toxin from the extracellular medium. omega-Agatoxin IVA (IVA; 100 nM) produced a 15% blockade of IBa. omega-Conotoxin MVIIC (MVIIC; 5 microM) produced a 36% blockade of...Continue Reading

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Citations

Jun 8, 2006·Pflügers Archiv : European journal of physiology·E CarboneV Carabelli
Apr 30, 2008·Pflügers Archiv : European journal of physiology·Alberto Pérez-AlvarezAlmudena Albillos
Nov 10, 2010·Cellular and Molecular Neurobiology·Pedro Segura-ChamaArturo Hernández-Cruz
Mar 7, 1996·European Journal of Pharmacology·L GandíaR Borges
Oct 1, 1996·Journal of Autonomic Pharmacology·P M Lundy, R Frew
Nov 20, 2002·Annals of the New York Academy of Sciences·Robert ZannerChristian Prinz
May 15, 2012·Journal of Molecular Neuroscience : MN·Juliana M RosaLuis Gandía
Oct 16, 1999·The Journal of Physiology·T NagayamaS Satoh
May 23, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Damien J KeatingMichael L Roberts
Nov 16, 2010·Journal of Neurochemistry·Yanina D Alvarez, Fernando D Marengo
Jul 30, 2003·Biophysical Journal·V CarabelliA R Artalejo
Aug 14, 2012·Biochimica Et Biophysica Acta·Norbert Weiss, Gerald W Zamponi
Sep 28, 1998·The European Journal of Neuroscience·Z Y GaoB A Wolf
Mar 20, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tsuyoshi AkiyamaKenji Sunagawa
Sep 4, 2017·Pflügers Archiv : European journal of physiology·Carmen NanclaresLuis Gandía
Nov 20, 2002·Annals of the New York Academy of Sciences·Inmaculada Cuchillo-IbanezAntonio G Garcia
Nov 3, 2005·The European Journal of Neuroscience·Eva Andrés-MateosCarmen Montiel
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Robert ZannerChristian Prinz
Jul 6, 2014·American Journal of Physiology. Cell Physiology·José-Carlos Fernández-MoralesAntonio Miguel G de Diego
Dec 17, 2010·American Journal of Physiology. Cell Physiology·A HernándezA Hernández-Cruz
Nov 24, 1999·The American Journal of Physiology·C PrinzM Gratzl
Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Y FukushimaS Satoh
May 15, 2009·American Journal of Physiology. Cell Physiology·José C Fernández-MoralesAntonio M G de Diego
Nov 15, 1997·The American Journal of Physiology·G GengN Yamaguchi
Nov 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Pedro Segura-ChamaArturo Hernández-Cruz

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