Multiple conductance channel activity of wild-type and voltage-dependent anion-selective channel (VDAC)-less yeast mitochondria

Biophysical Journal
T A Lohret, Kathleen W Kinnally

Abstract

Yeast mitoplasts (mitochondria with the outer membrane stripped away) exhibit multiple conductance channel activity (MCC) in patch-clamp experiments that is very similar to the activity previously described in mammalian mitoplasts. The possible involvement of the voltage-dependent anion-selective channel (VDAC) of the outer membrane in MCC activity was explored by comparing the channel activity in wild-type yeast mitoplasts with that of a VDAC-deletion mutant. The channel activity recorded from the mutant is essentially the same as that of the wild-type in the voltage range of -40 to 30 mV. These observations indicate that VDAC is not required for MCC activity. Interestingly, the channel activity of the VDAC-less yeast mitoplasts exhibits altered gating properties at transmembrane potentials above and below this range. We conclude that the deletion of VDAC somehow results in a modification of MCC's voltage dependence. In fact, the voltage profile recorded from the VDAC-less mutant resembles that of VDAC.

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Citations

Apr 1, 1996·Journal of Bioenergetics and Biomembranes·C A Mannella
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·K W KinnallyC A Mannella
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·C Ballarin, M C Sorgato
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Apr 30, 2003·Biophysical Journal·Concepción MuroMaría Luisa Campo
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Oct 25, 2006·Annual Review of Physiology·Brian O'Rourke
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Sep 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth A JonasLeonard K Kaczmarek
Nov 19, 2005·Biochimica Et Biophysica Acta·Evgeny PavlovKathleen W Kinnally
Jan 24, 2007·Analytical Biochemistry·Sonia Martinez-CaballeroMaría Luisa Campo
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