Multiple dengue virus serotypes resistant transgenic Aedes aegypti fitness evaluated under laboratory conditions.

RNA Biology
Hewawasam Patuwatha Badathuruge Kalindu Dulanja RamyasomaYasanthi Illika Nilmini Silva Gunawardene

Abstract

Dengue viruses (DENV) are the wildest transmitted arbovirus members of the family Flaviviridae, genus Flavivirus. Dengue viruses are composed of four serotypes, DENV1, 2, 3, and 4, and these viruses can cause dengue fever and dengue haemorrhagic fever or dengue shock syndrome, when infecting humans. RNA interference (RNAi) is a self-defence mechanism, which can be used to prevent invasions of RNA viruses to the host. Genetically engineering a host with an RNAi molecule that targets a single virus serotype may develop escape mutants, and can cause unusual dominance over other serotypes. Therefore, the simultaneous targeting of multiple serotypes is necessary to block DENV transmission. Here, we report the development of transgenic Aedes aegypti based on a bioinformatically designed multiple miRshRNA (microRNA-based shRNA) DNA sequence under the control of a blood-meal induced promoter, Carboxypeptidase A, to induce RNAi for DENV in Aedes aegypti, and demonstrate the expression of a synthetic multiple shRNA polycistronic cluster having RNA interference sequences to target DENV genomes. The transgenic mosquitoes have lower rates of infection, dissemination, and transmission for DENV2 and DENV4 compared to wild mosquitoes, with a s...Continue Reading

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Citations

Dec 11, 2020·Developmental and Comparative Immunology·Chinmay V Tikhe, George Dimopoulos
Mar 26, 2021·BioMed Research International·Hewawasam Patuwatha Badathuruge Kalindu Dulanja RamyasomaRanil Samantha Dassanayake

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Datasets Mentioned

BETA
JN054256.1
KJ726664.1
KJ726662.1
KJ726663.1
KJ468234.1
KF955476.1
KF955474.1
MK861918

Methods Mentioned

BETA
transgenic
PCR
electrophoresis
Reverse

Software Mentioned

Excel
mfold
BLAST
GraphPad Prism5

Related Concepts

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