Multiple detection of food-borne pathogenic bacteria using a novel 16S rDNA-based oligonucleotide signature chip

Biosensors & Bioelectronics
Ho Seop EomHyung Joon Cha

Abstract

There have been many attempts to develop sensitive and accurate techniques for the detection and diagnosis of pathogenic bacteria using nucleic acid-based technology. To achieve efficient multiple detection of seven selected food-borne pathogens, we assessed the respective 16S rDNA pathogen specific sequences using an oligonucleotide-based signature array. Strategic optimal design of specific capture probes was achieved by using the characteristic first variable region. To assess the specificity of this pathogen detection system, we employed a two-step experimental strategy. Under conditions established through experiments with chemically synthesized model targets comprising both conserved and variable regions of 16S rDNA, we confirmed the validity of this system using real 16S rDNA targets. Detection with real targets was successfully performed using our system, and better specificity was obtained compared to experiments with model targets. Moreover, the subtypes of Vibrio pathogens were successfully classified. We developed a two-dimensional visualization plot tool for positive control and specific spots, which allowed facile and minute differentiation between spot intensities. Repeated array formats were employed to ensure e...Continue Reading

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Citations

Nov 26, 2009·Applied Biochemistry and Biotechnology·Byeong Hee HwangHyung Joon Cha
Feb 5, 2008·Applied and Environmental Microbiology·Sarah M MillerSyed A Hashsham
Mar 31, 2010·Journal of Clinical Microbiology·Quan WangLei Wang
Dec 9, 2008·The Science of the Total Environment·Pedro AlbuquerqueFernando Tavares
Jan 22, 2010·Biotechnology and Bioengineering·Byeong Hee Hwang, Hyung Joon Cha
Sep 11, 2008·Analytical Biochemistry·Gi Won ShinGyoo Yeol Jung
Apr 21, 2009·Molecular and Cellular Probes·Seung Min YooJun Yong Choi
May 19, 2009·Current Opinion in Microbiology·Johanna Maukonen, Maria Saarela
Jan 17, 2020·Biotechnology Journal·Woogi Lee, Byeong Hee Hwang

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