Multiple developmental requirements of noisette, the Drosophila homolog of the U2 snRNP-associated polypeptide SP3a60.

Molecular and Cellular Biology
V MeyerD Pauli

Abstract

We report the cloning of the noisette gene (noi), which encodes the Drosophila melanogaster ortholog of a U2 snRNP-associated splicing factor, SF3a60 (SAP61) in humans and PRP9p in Saccharomyces cerevisiae. Antibodies raised against human SF3a60 recognized NOI in flies, showing a nuclear localization in all the stages examined, including the embryo, the dividing cells of imaginal discs, and the larval polyploid nuclei. NOI is expressed in somatic and germinal cells of both male and female gonads. By mobilization of P transposons, we have generated a large number of noi mutations. Complete loss of function resulted in lethality at the end of embryogenesis, without obvious morphological defects. Hypomorphic alleles revealed multiple roles of noi for the survival and differentiation of male germ cells, the differentiation of female germ cells, and the development of several adult structures.

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P K GeyerM M Green
Aug 17, 1989·Nature·B S Baker
Jan 1, 1988·Biotechnology·V Pirrotta
Sep 20, 1988·Journal of Molecular Biology·N H Brown, F C Kafatos
Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·C C Dapples, R C King
Sep 24, 1983·Nucleic Acids Research·G M Rubin, A C Spradling
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·G LazarH M Goodman
Apr 1, 1995·Genes & Development·J S Sun, J L Manley
Nov 1, 1994·Molecular and Cellular Biology·H Z Ring, J T Lis
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M D ChiaraR Reed
Aug 1, 1996·Trends in Biochemical Sciences·J Valcárcel, M R Green
Jan 1, 1996·Annual Review of Biochemistry·A Krämer
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·D Z RudnerD C Rio

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