Multiple developmental roles of VEGF suggested by a LacZ-tagged allele

Developmental Biology
L MiquerolA Nagy


Vascular endothelial growth factor (VEGF) is an angiogenic factor and a potent stimulator of microvascular permeability. It is a mitogen specific for endothelial cells. The expression of VEGF and its two receptors, Flk-1 and Flt-1, is pivotal for the proper formation of blood vessels in embryogenesis as shown by gene-targeting experiments. Interestingly, the loss of even a single allele of VEGF led to embryonic lethality between day E9.5 and day E10.5 in the mouse. To assess the role of VEGF during embryonic development we decided to tag VEGF expression with LacZ, by inserting an IRES (internal ribosome entry site)-LacZ reporter cassette into the 3' untranslated region of the gene. This alteration enabled us to monitor VEGF expression throughout embryonic development at single-cell resolution. beta-Galactosidase expression from the altered VEGF locus was first observed prior to gastrulation and was detectable at all stages of vascular development in the embryo. Later, the specific cellular distribution and the level of VEGF expression indicated its pleiotropic role in development. High expression levels seemed to be associated with vasculogenesis and permeability, whereas lower levels were associated with angiogenesis and cell ...Continue Reading


Sep 14, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B L Roman, B M Weinstein
Oct 15, 2009·Journal of Neural Transmission·Ronen Robert LekerDavid Chernoguz
Jul 6, 2000·The American Journal of Pathology·J KishimotoR E Burgeson
Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kartik BalachandranKevin Kit Parker
May 27, 2010·The Journal of Biological Chemistry·Jianyin LongFarhad R Danesh
Nov 13, 2002·The Journal of Biological Chemistry·Ehrin N JohnsonJoyce Bischoff
Feb 13, 2008·The Journal of Experimental Medicine·Arindel S R MaharajP A D'Amore
Jun 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jonathan T Butcher, R R Markwald
Oct 28, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Margaret A SchwarzHaihua Zheng
Sep 4, 2004·Circulation Research·Ehrin J Armstrong, Joyce Bischoff
May 11, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Ionela IacobasKaren K Hirschi
Aug 8, 2009·Circulation Research·Michelle D Combs, Katherine E Yutzey
Jun 9, 2012·Circulation Research·Alexander von Gise, William T Pu
Oct 20, 2012·Investigative Ophthalmology & Visual Science·Knatokie M FordP A D'Amore
Apr 9, 2005·Pediatric Research·David WarburtonWei Shi
Mar 9, 2012·Journal of Cell Science·Nathaniel G dela PazP A D'Amore
Feb 8, 2013·Biomarkers in Medicine·Michael S MageeScott A Waldman
Apr 9, 2014·Canadian Journal of Physiology and Pharmacology·Kiranj K Chaudagar, Anita A Mehta
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Tamar Licht, E Keshet
Feb 20, 2003·The Journal of Cell Biology·Josephine M EncisoJoseph A Madri
Apr 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sophie WiszniakQuenten Schwarz
Sep 2, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Amanda C LaRueChristopher J Drake


Jun 1, 1988·Developmental Biology·S M LoboG Schatten
May 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D J DumontM L Breitman
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K G PetersL T Williams
May 15, 1995·Genes & Development·T TakeuchiT Higashinakagawa
Jul 1, 1995·Circulation Research·L M Eisenberg, R R Markwald
Dec 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H G AugustinR C Johnson
Sep 1, 1993·Cancer Metastasis Reviews·D R SengerH F Dvorak
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A NagyJ C Roder
Jan 1, 1995·Annual Review of Cell and Developmental Biology·W Risau, I Flamme
Dec 27, 1996·Cell·J Folkman, P A D'Amore
Apr 17, 1997·Nature·W Risau
Mar 1, 1997·Developmental Biology·B FouquetM C Fishman
Mar 21, 1998·The Journal of Cell Biology·S EsserW Risau
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·S HiratsukaM Shibuya
Sep 15, 1998·Kidney International. Supplement·W Risau
Sep 15, 1998·Kidney International. Supplement·D R AbrahamsonT O Daniel
Oct 21, 1998·Current Biology : CB·F Dieterlen-Lièvre

Related Concepts

Untranslated Regions
Angiogenic Process
Chimeric Proteins, Recombinant
Entire Embryo
Embryonic Induction
Cardiovascular System
Blood Vessel
Trophic Factor Receptors

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