PMID: 33210590Nov 20, 2020Paper

Multiple differentiation potential of human periodontal ligament stem cells expressing stromal cell antigen 1 (STRO-1) sorted by MACS

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ying ZhaoMin Zhang

Abstract

Objective To perform in vitro characterization and comparison of stromal cell antigen 1 (STRO-1) positive (STRO-1+) and negative (STRO-1-) cell populations from human periodontal ligament cells sorted by magnetic-activated cell sorting (MACS). Methods Periodontal ligament cells were isolated from the periodontal ligament by tissue digesting method. The cells were magnetically sorted using anti-STRO-1 antibodies. Subsequently, cells were characterized by flow cytometry for the expression of CD90, CD105, CD29, STRO-1, CD34 and CD45. Colony-forming assay was used to detect the ability of colony-forming. When the cells are induced to differentiate by osteogenesis and lipogenesis, alizarin red staining, and oil red O staining were used to identify the pluripotent differentiation of STRO-1+ and STRO-1- cells. Results STRO-1+ cells highly expressed CD105 and CD90 and lowly expressed the haematopoietic markers CD45 and CD34. In contrast to STRO-1- cells, STRO-1+ cells showed a significantly higher number of colony-forming units (CFUs). STRO-1+ cells were demonstrated to have osteogenic and adipogenic differentiation potential. Conclusion STRO-1+ cells isolated by MACS are human periodontal ligament stem cells with stem cell characteris...Continue Reading

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