Multiple digit formation in Xenopus limb bud recombinants

Developmental Biology
H YokoyamaHiroyuki Ide

Abstract

We prepared recombinant limb buds of Xenopus tadpoles by grafting a mesenchyme mass of the hindlimb bud. The Xenopus recombinant limb buds with dissociated and reaggregated mesenchyme developed more than 30 digits with cartilage segmentation, while those with undissociated mesenchyme developed a limb with normal cartilage pattern. Before the formation of multiple digits, a patchy expression pattern of fgf-8, an AER marker, was observed in the distal region of recombinant limb buds. shh, a ZPA (zone of polarizing activity) marker, was expressed broadly in the distal region of recombinants. Recombinant limb buds with the reaggregated mesenchyme of anterior halves formed anterior digits with claws, and those with the mesenchyme of posterior halves formed posterior digits without claws. The temporal and spatial changes in the potency of multiple digit formation are discussed with reference to the regenerative capacity of Xenopus limb buds.

References

Feb 1, 1977·Developmental Biology·J A Cameron, J F Fallon
Jul 1, 1988·The Journal of Experimental Zoology·S K Sessions, S V Bryant
Oct 28, 1994·Biochemical and Biophysical Research Communications·H OhuchiS Noji
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Jun 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T EndoH Ide

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Citations

May 15, 2012·In Vitro Cellular & Developmental Biology. Animal·Keiko Shimizu-NishikawaAkio Nishikawa
Jan 24, 2008·Development, Growth & Differentiation·Yuka TaniguchiMakoto Mochii
Apr 11, 2013·Science Signaling·Takashi Miura
Nov 28, 2013·International Journal of Endocrinology·Ewa MatuszczakWojciech Debek
Apr 20, 2007·Developmental Biology·Hitoshi YokoyamaRandall T Moon
Dec 26, 2001·The Journal of Experimental Zoology·J HankenM W Klymkowsky
Nov 1, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Akira SatohHiroyuki Ide
Mar 16, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Akira SatohKoji Tamura
Jun 23, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nayuta YakushijiKoji Tamura
Nov 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hitoshi YokoyamaHiroyuki Ide

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