Multiple displacement amplification to create a long-lasting source of DNA for genetic studies

Human Mutation
Lovisa Lovmar, Ann-Christine Syvänen

Abstract

In many situations there may not be sufficient DNA collected from patient or population cohorts to meet the requirements of genome-wide analysis of SNPs, genomic copy number polymorphisms, or acquired copy number alternations. When the amount of available DNA for genotype analysis is limited, high performance whole-genome amplification (WGA) represents a new development in genetic analysis. It is especially useful for analysis of DNA extracted from stored histology slides, tissue samples, buccal swabs, or blood stains collected on filter paper. The multiple displacement amplification (MDA) method, which relies on isothermal amplification using the DNA polymerase of the bacteriophage phi29, is a recently developed technique for high performance WGA. This review addresses new trends in the technical performance of MDA and its applications to genetic analyses. The main challenge of WGA methods is to obtain balanced and faithful replication of all chromosomal regions without the loss of or preferential amplification of any genomic loci or allele. In multiple comparisons to other WGA methods, MDA appears to be most reliable for genotyping, with the most favorable call rates, best genomic coverage, and lowest amplification bias.

References

Oct 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xinghua PanSherman M Weissman
Mar 10, 2012·Science·Yoshiyuki ShibataAnindya Dutta
Jul 17, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Julie M CunninghamEllen L Goode
Dec 10, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Farzana JasmineMuhammad G Kibriya
Apr 16, 2009·BMC Genomics·Emiliano GiardinaGiuseppe Novelli
Sep 17, 2008·BMC Genomics·Muhammad ShoaibVasily Ogryzko
Jun 6, 2009·BMC Medical Genetics·Gary M ShawRichard H Finnell
Feb 9, 2008·BMC Medical Genetics·Samantha MaraghCatherine D O'Connell
Nov 26, 2011·Investigative Genetics·Eva C BerglundAnn-Christine Syvänen
Dec 2, 2008·Clinical Chemistry·Michael H ChoEdwin K Silverman
Jul 5, 2008·Emerging Infectious Diseases·Dana C CrawfordJairam Lingappa
Apr 22, 2009·International Journal of Biological Sciences·Zhihua JiangRichard M Harland
Sep 14, 2011·International Journal of Biological Sciences·Zhihua JiangRichard M Harland
May 12, 2009·Molecular and Biochemical Parasitology·Claudia L L ValentimCharles D Criscione
Apr 12, 2008·The Journal of Molecular Diagnostics : JMD·Daniel T CroftDarrell L Ellsworth
Aug 11, 2007·The Journal of Molecular Diagnostics : JMD·Fengfei WangG Mike Makrigiorgos
Oct 24, 2007·Analytical Biochemistry·Lin ChenPhilip J R Day
Jul 3, 2007·Journal of Neuroscience Methods·Kazuya IwamotoTadafumi Kato
Nov 21, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Espen MelumBenedicte A Lie
Mar 5, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Amir MoghaddamOlav Dalgard
Jan 27, 2015·Molecular Ecology Resources·Christopher BlairAnne D Yoder
Jan 5, 2011·Journal of Zhejiang University. Science. B·Ying-ming ZhengFan Jin
May 17, 2011·Forensic Science International. Genetics·Courtney M TateBruce Budowle
Nov 24, 2012·Clinical Epigenetics·Miki BundoKazuya Iwamoto
Aug 3, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Hege K PihlstrømEspen Melum
Nov 21, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Hege K PihlstrømEspen Melum

Citations

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L ZhangN Arnheim
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D L NelsonC T Caskey
Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Apr 12, 1996·The Journal of Biological Chemistry·L Blanco, M Salas
Oct 1, 1996·Genome Research·C A HeidP M Williams
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R LucitoM Wigler
Nov 4, 1998·Nucleic Acids Research·J BanérU Landegren
Jan 23, 1999·The American Journal of Pathology·W DietmaierJ Rüschoff
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·C A KleinG Riethmüller
Dec 6, 2001·Nature Reviews. Genetics·A C Syvänen
Jan 10, 2002·Analytical Biochemistry·Ralf KittlerManfred Kayser
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Frank B DeanRoger S Lasken
Sep 26, 2002·American Journal of Human Genetics·Michael CullenMary Carrington
Mar 26, 2003·Insect Molecular Biology·N Gorrochotegui-Escalante, W C Black
Apr 16, 2003·Genome Research·Seiyu HosonoRoger S Lasken
Dec 3, 2003·Environmental Microbiology·Karen E Nelson
Dec 24, 2003·Analytical Biochemistry·K J SorensenA T Christian
Mar 23, 2004·Genes, Chromosomes & Cancer·Denis A SmirnovVivian G Cheung
Jul 31, 2004·BioTechniques·Young M Kwon, Mandy M Cox
Jul 31, 2004·BioTechniques·Jin YanSteve S Sommer
Sep 2, 2004·Cold Spring Harbor Symposia on Quantitative Biology·J B FanM S Chee
Oct 7, 2004·Molecular Human Reproduction·Ali HellaniPinar Ozand

Related Concepts

Gene Dosage
Histology Procedure
Blood Stains
Genome
Genetic Analysis
Genetic Screening Method
Buccal Surface
Bacteriophages
Nucleic Acid Amplification Tests
Gene Amplification Technique

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