Multiple divergent picobirnaviruses with functional prokaryotic Shine-Dalgarno ribosome binding sites present in cloacal sample of a diarrheic chicken

Virology
Ákos BorosGábor Reuter

Abstract

Picobirnaviruses (PBVs) of family Picobirnaviridae have bisegmented (S1 and S2 segments), double-stranded RNA genomes. In this study a total of N = 12 complete chicken PBVs (ChPBV) segments (N = 5 of S1 and N = 7 of S2, Acc. Nos.: MH425579-90) were determined using viral metagenomic and RT-PCR techniques from a single cloacal sample of a diarrheic chicken. The identified ChPBV segments are unrelated to each other and distant from all of the currently known PBVs. In silico sequence analyses revealed the presence of conserved prokaryotic Shine-Dalgarno-like (SD-like) sequences upstream of the three presumed open reading frames (ORFs) of the S1 and a single presumed ORF of the S2 segments. According to the results of expression analyses in E. coli using 6xHis-tagged recombinant ChPBV segment 1 construct and Western blot these SD-like sequences are functional in vivo suggesting that S1 of study PBVs can contain three ORFs and supporting the bacteriophage-nature of PBVs.

Citations

Nov 30, 2018·MBio·Yuri I WolfEugene V Koonin
Mar 7, 2020·Microbiology and Molecular Biology Reviews : MMBR·Eugene V KooninJens H Kuhn
Sep 25, 2020·Viruses·Sergio Guajardo-LeivaBeatriz Díez
Jan 23, 2020·Viruses·Kirsty T T KwokMarion P G Koopmans
Dec 22, 2020·Avian Diseases·Sunil K MorRobert E Porter
Feb 9, 2021·Frontiers in Veterinary Science·Souvik Ghosh, Yashpal S Malik
Mar 5, 2021·Vavilovskiĭ Zhurnal Genetiki I Selekt︠s︡ii·A Yu KashnikovN A Novikova

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