Multiple DNA Interactions Contribute to the Initiation of Telomerase Elongation

Journal of Molecular Biology
Ahu Karademir AnderssonMarita Cohn

Abstract

Telomerase maintains telomere length and chromosome integrity by adding short tandem repeats of single-stranded DNA to the 3' ends, via reverse transcription of a defined template region of its RNA subunit. To further understand the telomerase elongation mechanism, we studied the primer utilization and extension activity of the telomerase from the budding yeast Naumovozyma castellii (Saccharomyces castellii), which displays a processive nucleotide and repeat addition polymerization. For the efficient initiation of canonical elongation, telomerase required 4-nt primer 3' end complementarity to the template RNA. This DNA-RNA hybrid formation was highly important for the stabilization of an initiation-competent telomerase-DNA complex. Anchor site interactions with the DNA provided additional stabilization to the complex. Our studies indicate three additional separate interactions along the length of the DNA primer, each providing different and distinct contributions to the initiation event. A sequence-independent anchor site interaction acts immediately adjacent to the base-pairing 3' end, indicating a protein anchor site positioned very close to the catalytic site. Two additional anchor regions further 5' on the DNA provide seque...Continue Reading

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Oct 30, 2016·Yeast·Ahu Karademir Andersson, Marita Cohn

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Citations

Aug 21, 2019·G3 : Genes - Genomes - Genetics·Marita CohnMirja Carlsson Möller
Feb 24, 2018·Nucleic Acids Research·Shankar ShastryMichael D Stone
Dec 16, 2021·Nucleic Acids Research·Humberto ItriagoMarita Cohn

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