Multiple effects of antibiotics on chloroplast and nuclear gene expression

Functional Plant Biology : FPB
Paula MuloEva-Mari Aro

Abstract

Antibiotics are widely used to monitor signalling cascades within a plant cell, for example between the nucleus and chloroplasts, and to study the function of the photosynthetic machinery. In the present study, we attempted to test various antibiotics with respect to their expected modes of function and also to monitor their possible side effects on metabolic processes in mature leaves of pea (Pisum sativum L.). Streptomycin, despite its reported prokaryotic nature, prevented translation not only in the chloroplast, but also in the cytosol. Application of puromycin, an inhibitor of protein synthesis in both the pro- and eukaryotes, resulted in severe photoinhibition of photosystem II upon illumination, yet had no effect on plastid translation, thus implying a severe side effect on plastid metabolism. Prokaryotic-type translation inhibitors lincomycin, spectinomycin and erythromycin blocked translation in the chloroplast without any direct effects on cytoplasmic protein synthesis. More detailed studies with lincomycin, however, revealed a strong modulation of the expression of nuclear-encoded genes by slowing down the transcription rate of photosynthesis-related Lhcb and RbcS genes, and furthermore, lincomycin clearly decreased ...Continue Reading

Citations

May 22, 2004·Physiologia Plantarum·Luke HendricksonWah Soon Chow
Nov 20, 2012·Antioxidants & Redox Signaling·Maija SierlaSaijaliisa Kangasjärvi
Sep 14, 2012·Plant Cell Reports·Rajesh Kumar TewariMasami Watanabe
Sep 9, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Xu WangJohan Auwerx
Apr 7, 2009·Plant, Cell & Environment·Saijaliisa KangasjärviEva-Mari Aro
Mar 5, 2015·Plant, Cell & Environment·Erica BelgioAlexander V Ruban
Aug 31, 2010·Plant & Cell Physiology·Isabel EgeaJean-Claude Pech
Sep 20, 2011·Journal of Experimental Botany·Tomohiro KakizakiTakehito Inaba
Feb 26, 2020·Physiologia Plantarum·James B Y H BehrendorffMathias Pribil
Oct 22, 2020·Cells·Luca TadiniPaolo Pesaresi
Oct 10, 2009·Plant Signaling & Behavior·Eevi RintamäkiSaijaliisa Kangasjärvi
Mar 3, 2012·Plant Physiology·Michael E RuckleRobert M Larkin
May 23, 2021·Nature Communications·Chuanyang YuWilson Chun Yu Lau
May 15, 2021·Journal of Experimental Botany·Vesa HavurinneEsa Tyystjärvi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Internationale Zeitschrift für klinische Pharmakologie, Therapie, und Toxikologie. International journal of clinical pharmacology, therapy, and toxicology
W J HOLLOWAY
© 2021 Meta ULC. All rights reserved