Multiple effects of genetic background on variegated transgene expression in mice.

Genetics
Margaret L OpsahlC B Whitelaw

Abstract

BLG/7 transgenic mice express an ovine beta-lactoglobulin transgene during lactation. Unusually, transgene expression levels in milk differ between siblings. This variable expression is due to variegated transgene expression in the mammary gland and is reminiscent of position-effect variegation. The BLG/7 line was created and maintained on a mixed CBA x C57BL/6 background. We have investigated the effect on transgene expression of backcrossing for 13 generations into these backgrounds. Variable transgene expression was observed in all populations examined, confirming that it is an inherent property of the transgene array at its site of integration. There were also strain-specific effects on transgene expression that appear to be independent of the inherent variegation. The transgene, compared to endogenous milk protein genes, is specifically susceptible to inbreeding depression. Outcrossing restored transgene expression levels to that of the parental population; thus suppression was not inherited. Finally, no generation-dependent decrease in mean expression levels was observed in the parental population. Thus, although the BLG/7 transgene is expressed in a variegated manner, there was no generation-associated accumulated silenc...Continue Reading

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Citations

Feb 21, 2006·Experimental Neurology·Felix P EckensteinJason Deignan
Feb 12, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sang Su ShinJae Yong Han
Oct 26, 2005·Transgenic Research·David M DonovanRobert J Wall
Sep 3, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Aurora Ruiz-HerreraTerence J Robinson
Mar 4, 2011·Reproduction, Fertility, and Development·Jorge A Piedrahita, Natasha Olby

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