PMID: 7932644Jun 1, 1994Paper

Multiple effects of protein kinase C activators on Na+ currents in mouse neuroblastoma cells

The Journal of Membrane Biology
C M Godoy, S Cukierman


The effects of externally applied different protein kinase C (PKC) activators on Na+ currents in mouse neuroblastoma cells were studied using the perforated-patch (nystatin-based) whole cell voltage clamp technique. Two diacylglycerol-like compounds, OAG (1-oleoyl-2-acetyl-sn-glycerol), and DOG (1-2-dioctanoyl-rac-glycerol) attenuated Na+ currents without affecting the time course of activation or inactivation. The reduction in Na+ current amplitude caused by OAG or DOG was dependent on membrane potential, being more intense at positive voltages. The steady-state activation curve was also unaffected by these substances. However, both OAG and DOG shifted the steady-state inactivation curve of Na+ currents to more hyperpolarized voltages. Surprisingly, phorbol esters did not affect Na+ currents. Cis-unsaturated fatty acids (linoleic, linolenic, and arachidonic) attenuated Na+ currents without modifying the steady-state activation. As with DOG and OAG, cis-unsaturated fatty acids also shifted the steady-state inactivation curve to more negative voltages. Interestingly, inward currents were more effectively attenuated by cis-fatty acids than outward currents. Oleic acid, also a cis-unsaturated fatty acid, enhanced Na+ currents. Thi...Continue Reading


Dec 1, 1994·Pflügers Archiv : European journal of physiology·C M Godoy, S Cukierman
Oct 23, 1997·The Journal of Physiology·J P O'ReillyG G Haddad
Feb 13, 2001·Experimental Neurology·M E EhrlichE Cattaneo
Mar 5, 1998·The American Journal of Physiology·K B WalshJ Fan

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