Multiple elements required for translation of plastid atpB mRNA lacking the Shine-Dalgarno sequence

Nucleic Acids Research
Tetsuro Hirose, Masahiro Sugiura

Abstract

The mechanism of translational initiation differs between prokaryotes and eukaryotes. Prokaryotic mRNAs generally contain within their 5'-untranslated region (5'-UTR) a Shine-Dalgarno (SD) sequence that serves as a ribosome-binding site. Chloroplasts possess prokaryotic-like translation machinery, and many chloroplast mRNAs have an SD-like sequence, but its position is variable. Tobacco chloroplast atpB mRNAs contain no SD-like sequence and are U-rich in the 5'-UTR (-20 to -1 with respect to the start codon). In vitro translation assays with mutated mRNAs revealed that an unstructured sequence encompassing the start codon, the AUG codon and its context are required for translation. UV crosslinking experiments showed that a 50 kDa protein (p50) binds to the 5'-UTR. Insertion of an additional initiation region (SD-sequence and AUG) in the 5'-UTR, but not downstream, arrested translation from the authentic site; however, no inhibition was observed by inserting only an AUG triplet. We hypothesize for translational initiation of the atpB mRNA that the ribosome enters an upstream region, slides to the start codon and forms an initiation complex with p50 and other components.

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Citations

Jul 31, 2007·Photosynthesis Research·Julia Marín-NavarroStephen P Mayfield
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Maki Yukawa, Masahiro Sugiura
Oct 26, 2007·Nucleic Acids Research·Alexandra-Viola BohneRalph Bock
Oct 23, 2010·Nucleic Acids Research·Oliver Drechsel, Ralph Bock
Aug 27, 2009·BMC Genomics·Diana L WilliamsThomas P Gillis
Aug 9, 2007·PLoS Biology·Andrea L ManuellStephen P Mayfield
Apr 9, 2014·Molecular Biology and Evolution·Kyungtaek LimKenta Nakai
Jun 20, 2012·The Plant Journal : for Cell and Molecular Biology·Reimo ZoschkeChristian Schmitz-Linneweber
May 30, 2012·The Plant Journal : for Cell and Molecular Biology·Jiang ZhangRalph Bock
Sep 26, 2012·The Plant Journal : for Cell and Molecular Biology·Rodrigo CarocaRalph Bock
Mar 5, 2014·Molecular Plant·Nadine Tiller, Ralph Bock

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