Multiple-endpoint assay provides a detailed mechanistic view of responses to herbicide exposure in Chlamydomonas reinhardtii

Aquatic Toxicology
Holger NestlerMarc J-F Suter

Abstract

The release of herbicides into the aquatic environment raises concerns about potential detrimental effects on ecologically important non-target species, such as unicellular algae, necessitating ecotoxicological risk assessment. Algal toxicity tests based on growth, a commonly assessed endpoint, are integrative, and hence do not provide information about underlying toxic mechanisms and effects. This limitation may be overcome by measuring more specific biochemical and physiological endpoints. In the present work, we developed and applied a novel multiple-endpoint assay, and analyzed the effects of the herbicides paraquat, diuron and norflurazon, each representing a specific mechanism of toxic action, on the single celled green alga Chlamydomonas reinhardtii. The endpoints added to assessment of growth were pigment content, maximum and effective photosystem II quantum yield, ATP content, esterase and oxidative activity. All parameters were measured at 2, 6 and 24h of exposure, except for growth and pigment content, which were determined after 6 and 24h only. Effective concentrations causing 50% of response (EC50s) and lowest observable effect concentrations (LOECs) were determined for all endpoints and exposure durations where po...Continue Reading

References

Aug 12, 2000·Journal of Experimental Botany·K Maxwell, G N Johnson
Dec 1, 2004·Annual Review of Genetics·Arthur R GrossmanChung Soon Im
Jul 30, 2005·Environmental Toxicology and Chemistry·Séverine Le FaucheurLaura Sigg
Feb 18, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Grzegorz Bartosz
Jul 31, 2007·Plant, Cell & Environment·Dominique RumeauLaurent Cournac
Aug 24, 2007·Environmental Toxicology and Chemistry·Nicole E AdlerRolf Altenburger
Sep 26, 2009·Toxicology Mechanisms and Methods·Annick HourmantFrançoise Quiniou
Apr 1, 2008·Journal of Phycology·Stefanie Knauert, Katja Knauer

❮ Previous
Next ❯

Citations

Mar 13, 2014·Journal of Photochemistry and Photobiology. B, Biology·Enrique NavarroRenata Behra
Feb 11, 2014·Ecotoxicology and Environmental Safety·Stephka G ChankovaNadezhda P Yurina
Dec 24, 2013·Environment International·Muhammad Zaffar HashmiChaofeng Shen
Jan 19, 2016·Pesticide Biochemistry and Physiology·Michael MoustakasIlektra Sperdouli
Aug 15, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Giulia Cheloni, Vera I Slaveykova
Sep 22, 2015·Environmental Toxicology and Chemistry·Vincent C ReyesShaily Mahendra
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Smitha PillaiKristin Schirmer
May 25, 2016·Environmental Pollution·Sun-Hwa Nam, Youn-Joo An
Jan 18, 2015·Current Opinion in Microbiology·Venu Kamarthapu, Evgeny Nudler
Jul 7, 2016·Environmental Science and Pollution Research International·Qiongzhi ZhouYangduo Kuang
Oct 28, 2019·Scientific Reports·Sreejith Kottuparambil, Jihae Park
Nov 14, 2019·International Journal of Environmental Research and Public Health·Agata Jabłońska-TrypućAndrzej Butarewicz
Dec 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Angela PannierMechthild Schmitt-Jansen
Dec 19, 2020·Aquatic Toxicology·Giulia Cheloni, Vera I Slaveykova
Apr 18, 2020·Ecotoxicology and Environmental Safety·Marta EsperanzaÁngeles Cid
Dec 11, 2020·Journal of Agricultural and Food Chemistry·Xin-Lin SunZhi-Qin Ji

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.