Multiple endpoints to evaluate pristine and remediated titanium dioxide nanoparticles genotoxicity in lung epithelial A549 cells

Toxicology Letters
Andrea StoccoroLucia Migliore

Abstract

Titanium dioxide nanoparticles (TiO2 NP) are broadly used in a wide range of applications. Several studies have reported that TiO2 NP possess cytotoxic and genotoxic properties that could induce adverse health effects in humans. The FP7 Sanowork project was aimed to minimize occupational hazard and exposure to engineered nanomaterials (ENM), including TiO2 NP, through the surface modification in order to avoid possible adverse toxic effects for humans. In this study we investigated cytotoxicity, genotoxicity and epigenetic properties of TiO2 NP uncoated and coated with silica or citrate, as well as of the benchmark material P25. We used a panel of in vitro assays in the human lung epithelial cell line A549, in order to better understand if the remediation strategy adopted was able to counteract possible toxic effects of uncoated TiO2 NP. Our results showed that the uncoated TiO2 NP were both cytotoxic and genotoxic, and the remediation strategy adopted did not reduce the adverse effects of uncoated TiO2 NP. In particular, the presence of citrate was able to increase their cytotoxicity and genotoxicity, exerting also epigenotoxic effects, as evaluated by the marked reduction of LINE-1 methylation levels.

Citations

May 24, 2018·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Amir AsghariSoghra Mehri
Mar 30, 2019·Nanoscale Research Letters·Valeria De MatteisRosaria Rinaldi
Nov 28, 2020·Archives of Toxicology·Nancy B Andrioli, Gabriela Chaufan
Jan 8, 2021·Journal of Nanobiotechnology·Marta Pogribna, George Hammons
Jul 15, 2020·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Marie CarriereThierry Douki
May 13, 2021·EFSA Journal·UNKNOWN EFSA Panel on Food Additives and Flavourings (FAF)Matthew Wright
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