Multiple epsilon-promoter elements participate in the developmental control of epsilon-globin genes in transgenic mice.

The Journal of Biological Chemistry
Qiliang LiG Stamatoyannopoulos

Abstract

To delineate the regulation of the human epsilon-globin gene, we investigated epsilon-gene expression during the development of transgenic mice carrying constructs with epsilon-promoter truncations linked to a micro-locus control region (microLCR). Expression levels were compared with those of microLCR epsilon mice carrying a 2 kilobase epsilon-promoter and betaYAC controls. epsilon mRNA in the embryonic cells of microLCR (-179)epsilon mice were as high as in microLCR epsilon mice suggesting that the proximal epsilon-promoter contains most elements required for epsilon-gene activation. epsilon mRNA in adult microLCR (-179) epsilon mice was significantly lower than in the embryonic cells indicating that elements involved in epsilon-gene silencing are contained in the proximal epsilon-promoter. Extension of the promoter sequence to -463 epsilon decreased epsilon-gene expression in the definitive erythroid cells, supporting previous evidence that the -179 to -463epsilon region contains an epsilon-gene silencer. However, the epsilon-gene of the microLCR(-463)epsilon mice was not silenced in the definitive cells of fetal and adult erythropoiesis indicating that additional silencing elements are located upstream of position -463epsil...Continue Reading

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Citations

Feb 26, 2005·Experimental Hematology·George Stamatoyannopoulos
Jul 4, 2002·Molecular Aspects of Medicine·Thomas Brittain
Aug 16, 2001·Molecular Biology and Evolution·J R Stone, G A Wray
Feb 8, 2006·PLoS Genetics·Zanhua YiMurray H Brilliant
Apr 9, 2018·Biochemistry. Biokhimii︠a︡·O V IarovaiaS V Ulianov
May 5, 2004·Genesis : the Journal of Genetics and Development·Kenneth R PetersonThalia Papayannopoulou
Feb 22, 2002·The Journal of Biological Chemistry·Heather A FoleyBetty S Pace
Apr 14, 2007·The EMBO Journal·Osamu TanabeJames Douglas Engel
Mar 4, 2006·Experimental Biology and Medicine·Patrick A NavasGeorge Stamatoyannopoulos
Sep 27, 2002·Experimental Biology and Medicine·Susanna HarjuKenneth R Peterson

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