Multiple excitation nano-spot generation and confocal detection for far-field microscopy

Nanoscale
Partha Pratim Mondal

Abstract

An imaging technique is developed for the controlled generation of multiple excitation nano-spots for far-field microscopy. The system point spread function (PSF) is obtained by interfering two counter-propagating extended depth-of-focus PSF (DoF-PSF), resulting in highly localized multiple excitation spots along the optical axis. The technique permits (1) simultaneous excitation of multiple planes in the specimen; (2) control of the number of spots by confocal detection; and (3) overcoming the point-by-point based excitation. Fluorescence detection from the excitation spots can be efficiently achieved by Z-scanning the detector/pinhole assembly. The technique complements most of the bioimaging techniques and may find potential application in high resolution fluorescence microscopy and nanoscale imaging.

References

Apr 6, 1990·Science·W DenkW W Webb
Mar 29, 2000·Biophysical Journal·G H Patterson, D W Piston
Dec 17, 2003·Microscopy Research and Technique·Alessandro EspositoAlberto Diaspro
May 26, 2007·Science·Stefan W Hell
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Partha Pratim Mondal, Alberto Diaspro
Oct 2, 2009·The Review of Scientific Instruments·Partha Pratim Mondal

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Citations

Jun 10, 2014·PloS One·Kavya MohanPartha Pratim Mondal
Feb 23, 2012·Scientific Reports·Partha Pratim Mondal, Alberto Diaspro

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