PMID: 9188565Jul 1, 1997Paper

Multiple extracellular domains of CCR-5 contribute to human immunodeficiency virus type 1 entry and fusion

Journal of Virology
L PicardP R Clapham

Abstract

Human immunodeficiency virus type 1 (HIV-1) entry is governed by the interaction of the viral envelope glycoprotein (Env) with its receptor. The HIV-1 receptor is composed of two molecules, the CD4 binding receptor and a coreceptor. The seven-membrane-spanning chemokine receptor CCR-5 is one of the coreceptors used by primary isolates of HIV-1. We demonstrate that the mouse homolog of CCR-5 (mCCR-5) does not function as an HIV-1 coreceptor. A set of chimeras of human CCR-5 and mCCR-5 was studied for Env-induced cell fusion and HIV-1 infection. Using the HIV-1ADA envelope glycoprotein in a syncytium formation assay, we show that replacement of any fragment containing extracellular domains of mCCR-5 by its human counterparts is sufficient to allow Env-induced fusion. Conversely, replacement of any fragment containing human extracellular domains by its murine counterpart did not lead to coreceptor function loss. These results show that several domains of CCR-5 participate in coreceptor function. In addition, using a panel of primary nonsyncytium-inducing and syncytium-inducing isolates that use CCR-5 or both CXCR-4 and CCR-5 as coreceptors, we show that the latter dual-tropic isolates are less tolerant to changes in CCR-5 than str...Continue Reading

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Citations

Jun 5, 1999·AIDS Research and Human Retroviruses·F VerrierM Girard
Nov 21, 2001·British Medical Bulletin·P R Clapham, A McKnight
May 25, 2006·Journal of Molecular Modeling·Kelby B NapierJohn O Trent
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·T M Ross, B R Cullen
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·W K WangT H Lee
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·E G CormierT Dragic
Sep 10, 2002·AIDS·Julio Martín-GarcíaFrancisco González-Scarano
May 1, 2010·Viruses·Michael A LobritzEric J Arts
Oct 12, 2000·Journal of Virology·P D Bieniasz, B R Cullen
Jul 19, 2002·The Journal of General Virology·Paul R Clapham, Aine McKnight
Aug 3, 2002·The Journal of Biological Chemistry·Maurice Chelli, Marc Alizon
Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
May 25, 2002·The Journal of General Virology·Jacqueline D Reeves, Robert W Doms
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·B ChackerianJ T Schiller
Oct 16, 2001·The Journal of Biological Chemistry·M Chelli, M Alizon
Jan 9, 2010·AIDS Research and Human Retroviruses·John C TiltonRobert W Doms
May 14, 2011·Experimental Biology and Medicine·Won-Tak Choi, Jing An
May 20, 1999·Molecular Membrane Biology·P R ClaphamA McKnight
Apr 26, 2008·Journal of Receptor and Signal Transduction Research·Terry Kenakin

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