PMID: 3384332May 1, 1988Paper

Multiple factors are required for poly(A) addition to a mRNA 3' end

Genes & Development
M A McDevittJ R Nevins

Abstract

Polyadenylation of pre-mRNAs in the nucleus involves a specific endonucleolytic cleavage, followed by the addition of approximately 200 adenylic acid residues. We have assayed HeLa nuclear extracts for the activity that catalyzes the poly(A) addition reaction. The authenticity of the in vitro assay was indicated by the observation that the poly(A) tract added in vitro is approximately 200 nucleotides in length. We have fractionated nuclear extracts in order to define components involved in specific poly(A) addition. No single fraction from DEAE-Sephacel chromatography of a HeLa nuclear extract possessed the specific poly(A) addition activity. However, if the various fractions were recombined, activity was restored, indicating the presence of multiple components. Further fractionation revealed the presence of at least two factors necessary for the poly(A) addition reaction. The reconstituted system retains the characteristics and specificity seen in the crude extract. Additional purification of one of the factors strongly suggests it to be a previously characterized poly(A) polymerase which, when assayed in the absence of the other factor, can add AMP to an RNA terminus but without specificity. Thus, the other component of the r...Continue Reading

References

Jan 1, 1976·Progress in Nucleic Acid Research and Molecular Biology·M Edmonds, M A Winters
Dec 1, 1985·Arthritis and Rheumatism·D E FisherN Chiorazzi
Sep 1, 1987·Genes & Development·H Skolnik-DavidP A Sharp
Feb 13, 1987·Science·P A Sharp
Jan 1, 1983·Annual Review of Biochemistry·J R Nevins

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Citations

Jan 1, 1990·Molecular Biology Reports·R Lührmann
Sep 1, 1988·Trends in Genetics : TIG·T Humphrey, N J Proudfoot
Jun 26, 1989·Nucleic Acids Research·T A ZwierzynskiG A Buck
Dec 18, 1991·European Journal of Biochemistry·A AströmA Virtanen
Apr 1, 1989·Molecular and Cellular Biology·M P Terns, S T Jacob
Jan 1, 1990·Molecular and Cellular Biology·V J Bardwell, M Wickens
Feb 1, 1990·Molecular and Cellular Biology·V J BardwellM Wickens
Nov 1, 1988·Trends in Biochemical Sciences·K L Mowry, J A Steitz
Nov 1, 1989·Molecular and Cellular Biology·J D DeZazzo, M J Imperiale
Jan 1, 1991·Genes & Development·H SanfaçonT Hohn
Nov 1, 1989·Genes & Development·Y TakagakiJ L Manley
Sep 1, 1989·Genes & Development·M D Sheets, M Wickens

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