PMID: 76Nov 20, 1975

Multiple forms of casein kinase from rabbit erythrocytes

Biochimica Et Biophysica Acta
R Kumar, M Tao

Abstract

Two rabbit erythrocyte casein kinases, GTP:casein kinase I and GTP:casein kinase II, have been purified 29 000- and 47 000-fold, respectively. Studies employing sucrose density gradient centrifugation indicate that kinase I has a molecular weight of about 9.5 - 10(5) (25 S) and kinase II about 1.4 - 10(6) (32 S). These enzymes can utilize either ATP or GTP as the phosphoryl donor. Among various protein substrates examined, these kinases catalyze the phosphorylation of casein greater than 50% dephosphorylated phosvitin congruent to 50% dephosphorylated casein greater than phosvitin. Histones, protamine and bovine serum albumin are poor phosphoryl acceptors. Kinetic data indicate that both enzymes are inhibited by high casein substrate concentrations which may be partially relieved by NaCl. Both phosphotransferases require Mg(2+) for activity and are optimally active at pH 9.0. The enzymes have apparent Km values of 2.5 - 10(-5) M for GTP, 2 - 10(-5) M for ATP, and 0.4--0.6 mg/ml for casein. The incorporation of the terminal phosphate of GTP into casein as catalyzed by these enzymes is inhibited to varying degrees by ATP, ITP, ADP, and GDP but not by UTP, CTP, GMP, adenosine 3':5'-cyclic monophosphate, and guanosine 3':5'-cyclic ...Continue Reading

References

Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J A TrauchR R Traut
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·F A Ventimiglia, I G Wool

Citations

Oct 27, 2010·Cell and Tissue Research·Mathias Montenarh
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R BenneJ W Hershey
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S H MayrandT Pederson
Sep 10, 2014·Current Opinion in Cell Biology·Cristina-Maria Cruciat
Sep 16, 1981·Biochemical and Biophysical Research Communications·M C Lecomte, P Boivin
Feb 13, 2020·Cell Cycle·Luke J Fulcher, Gopal P Sapkota
Feb 29, 2020·International Journal of Molecular Sciences·Junmei Kang, Zhen Wang
Jun 7, 2018·Biochemical Society Transactions·Polyxeni Bozatzi, Gopal P Sapkota
Jan 1, 1985·American Journal of Hematology·R Kumar
Dec 12, 2020·The Biochemical Journal·Luke J Fulcher, Gopal P Sapkota
Dec 7, 2020·The Plant Journal : for Cell and Molecular Biology·Zhen WangHeriberto Cerutti

Related Concepts

Metazoa
Casein A
Enzyme Activation
Erythrocytes
Fluorides
Hydrogen-Ion Concentration
Alloenzymes
Magnesium
Protein KINASE
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