PMID: 2116165May 29, 1990Paper

Multiple forms of Go alpha mRNA: analysis of the 3'-untranslated regions

Biochemistry
S R PriceM Vaughan

Abstract

Go, a guanine nucleotide binding protein found predominantly in neural tissues, interacts in vitro with rhodopsin, muscarinic, and other receptors and has been implicated in the regulation of ion channels. Despite the virtual identity of reported cDNA sequences for the alpha subunit of Go (Go alpha), multiple molecular weight forms of mRNA have been identified in tissues from all species examined. To investigate the molecular basis for the size heterogeneity of Go alpha mRNAs, four cDNA clones were isolated from the same retinal lambda gt10 cDNA library that was used earlier to isolate lambda GO9, a clone encompassing the complete coding region of Go alpha. These clones were identified as Go alpha clones based on nucleotide sequence identity with lambda GO9 in the coding region; they diverge, however, from lambda GO9 in the 3'-untranslated region 28 nucleotides past the stop codon. An oligonucleotide probe complementary to a portion of the 3'-untranslated region of lambda GO9 that differs from the newly isolated clones hybridized with 3.0- and 4.0-kb mRNAs present in bovine brain and retina whereas a similar probe for the unique region of the new clones hybridized with a 4.0-kb mRNA in both tissues and with a 2.0-kb mRNA found ...Continue Reading

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Citations

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T TsukamotoY Kaziro
Oct 1, 1992·Molecular and Cellular Neurosciences·M A SmithU J McMahan
Aug 1, 1997·Biochemical Pharmacology·J D Hildebrandt
Feb 28, 1992·Biochemical and Biophysical Research Communications·T B HaugenV Hansson
Mar 3, 1995·The Journal of Biological Chemistry·M D WilcoxJ D Hildebrandt

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