Dec 1, 1975

Multiple forms of human glutathione S-transferase and their affinity for bilirubin

European Journal of Biochemistry
K KamisakaW B Jakoby


The initial enzymic step in mercapturic acid formation is catalyzed by glutathione S-transferase. Several species of this enzyme, designated as transferases alpha, beta, gamma, delta and epsilon on the basis of increasing isoelectric points, were isolated from human liver. Evidence is presented that each of the purified species is homogeneous with respect to sodium dodecylsulfate-gel electrophoresis. Transferases alpha, beta and epsilon each appear as a single band on gel electrofocusing; transferases gamma and delta are present as two and three bands, respectively, with each band catalytically active. Amino acid analysis indicated the five transferases to be either very closely related or identical in this respect. All enzyme species have a molecular weight of about 48500 and consist of two apparently identical subunits. The spectrum of substrates is the same for each although the enzymes differ slightly in specific activity. As is the case for the rat liver enzymes, each of the human transferases binds bilirubin although this compound is not a substrate.

Mentioned in this Paper

Enzymes, antithrombotic
Bilirubin, (4E,15E)-Isomer
Serum Bilirubin Measurement
Plasma Protein Binding Capacity
Blood Bilirubin Measurement
Enzymes for Treatment of Wounds and Ulcers
Binding (Molecular Function)

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