PMID: 3771097Aug 1, 1986Paper

Multiple forms of pituitary prolactin, a glycosylated form of porcine prolactin with enhanced biological activity

International Journal of Peptide and Protein Research
Pankov YuA, Butnev VYu

Abstract

Three forms of prolactin differing in molecular weight (Mr 23,000, 25,000 and 50,000, respectively) and electrophoretic mobility have been isolated and purified from fresh-frozen porcine pituitary glands. The prolactin form with Mr 25,000 is identified as a glycoprotein having an affinity to concanavalin A. The carbohydrate unit containing GlcNAc3, GalNAc1, Man3, Fuc 0.5, Gal 0.4 is linked to asparagine at the 31 position. The amino acid composition and partial primary structure of the glycosylated prolactin are identical to those of the major prolactin form (Mr 23,000). Based on isoelectric focusing and nondenaturing disc-electrophoresis, the glycosylated prolactin appears more acidic than the major form. The glycosylated form of the hormone has 140% of the activity of the non-glycosylated prolactin when measured by the pigeon crop sac assay. The new form accounts for 30-40% of the total monomeric prolactin in the porcine pituitary. The yield was 200 mg purified glycosylated prolactin from 1000 g pituitary gland. The third form of hormone (Mr 50,000) was shown to be a disulphide linked prolactin dimer.

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Citations

Jul 1, 1996·Journal of Protein Chemistry·V Y ButnevG R Bousfield
Mar 1, 1992·International Journal of Peptide and Protein Research·T NosoH Kawauchi
Dec 1, 1991·Journal of Endocrinological Investigation·M E FonsecaA Zárate
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Mar 1, 1992·General and Comparative Endocrinology·M Rand-Weaver, H Kawauchi
Jul 10, 1993·Biochimica Et Biophysica Acta·T NosoH Kawauchi
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D H Corcoran, J A Proudman
Jun 18, 1990·Molecular and Cellular Endocrinology·C L BrooksG C Johnson
Jun 18, 1990·Molecular and Cellular Endocrinology·K H YoungF W Bazer
Sep 1, 1991·Molecular and Cellular Endocrinology·Y N SinhaU J Lewis
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N MartinatY Combarnous
Apr 1, 1994·Nuclear Medicine and Biology·B ClaustratM O Joly
Jan 1, 1991·General and Comparative Endocrinology·M Rand-WeaverH Kawauchi
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Sep 30, 1988·Biochemical and Biophysical Research Communications·P R AtkinsonJ P Hughes
Jun 16, 2009·General and Comparative Endocrinology·Gen HiyamaDavid Zadworny

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