Multiple forms of selenoprotein P in a candidate human plasma standard reference material

Metallomics : Integrated Biometal Science
Guillaume BallihautW Clay Davis

Abstract

Selenoprotein P (SePP) is a unique selenium-containing protein responsible for the transport and distribution of the essential trace element selenium (Se) through the human body with the concentration of SePP in human blood representing the most useful marker of Se nutritional status. Although SePP has been extensively studied, the structure of SePP in human plasma remains unresolved. Two potential isoforms of SePP have been identified by Western blot analyses distinguished principally by differences in migration (51 kDa and 61 kDa). The biological relevance of the smaller isoform has been called into question by several studies reporting only one major SePP form (69 kDa) suggesting that the shorter 51 kDa is an artifact of protease activity during the SePP purification process. A deficiency of these Western blot analyses is that no information can be gleaned regarding the Se content of the potential isoforms. This study reports a characterization of SePP isoforms in a human plasma Standard Reference Material representative of a healthy US population. Following immunoprecipitation, three SePP isoforms were unequivocally identified at 45 kDa, 49 kDa and 57 kDa (termed as SePP45, SePP49 and SePP57) by LC-MS/MS analyses from a spe...Continue Reading

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Citations

Aug 8, 2012·Analytical and Bioanalytical Chemistry·Nikolay SolovyevBernhard Michalke
May 11, 2013·Analytical and Bioanalytical Chemistry·Claudia Swart
Dec 15, 2015·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Esther Humann-ZiehankBernhard Michalke
May 23, 2015·Metallomics : Integrated Biometal Science·Bárbara Rita CardosoDominic J Hare
May 26, 2015·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Marco VincetiBernhard Michalke
Nov 5, 2013·Metallomics : Integrated Biometal Science·Marco RomanCarlo Barbante
May 24, 2017·The British Journal of Nutrition·Cristina SantosInés Velasco
Mar 20, 2019·Shock·Petra BüttnerHolger Thiele
Jul 3, 2021·International Journal of Molecular Sciences·Jérémy LamarcheRyszard Lobinski

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