PMID: 820122Apr 7, 1975

Multiple forms of soluble monophenol, dihydroxyphenylalanine: oxygen-oxidoreductase (EC 1.14.18.1) from potato tubers (Solanum tuberosum)

Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung
G Matheis, H D Belitz

Abstract

Upon polyacrylamide gel electrophoresis, a soluble phenoloxidase from potatoes (var. Maritta) revealed 17 multiple forms with activity towards dopa and almost all other o-diphenols tested, but only 5 of the forms reacted with monophenols. Isoelectric focusing of the crude enzyme resulted in 2 main peaks with activity towards dopa, having isoelectric points at pH ranges 4.0-4.7 and 5.1-5.4: smaller amounts of the enzyme at higher pI values were also detected. When activity peaks were controlled by polyacrylamide gel electrophoresis, all bands previously detected by electrophoresis of the crude enzyme were recovered, but all peaks were electrophoretically heterogeneous. Gel chromatography of the crude enzyme showed different molecular forms. Their molecular weights indicated monomer, dimer, tetramer, octamer and polymer (at least hexadecamer) forms with a monomer molecular weight of about 36000.

References

May 1, 1966·Journal of Biochemistry·T NakamuraY Ogura
Jun 1, 1970·The Biochemical Journal·K Balasingam, W Ferdinand
May 6, 1967·Nature·R G Edwards, R L Gardner
Jul 9, 1962·Biochimica Et Biophysica Acta·A S FOX, J B BURNETT
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

Citations

Jan 1, 1981·Critical Reviews in Food Science and Nutrition·L Vámos-Vigyázó

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Catechol Oxidase
Alloenzymes
Vegetables

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