PMID: 9658177Jul 11, 1998Paper

Multiple functions for actin during filamentous growth of Saccharomyces cerevisiae

Molecular Biology of the Cell
B M CaliG R Fink

Abstract

Saccharomyces cerevisiae is dimorphic and switches from a yeast form to a pseudohyphal (PH) form when starved for nitrogen. PH cells are elongated, bud in a unipolar manner, and invade the agar substrate. We assessed the requirements for actin in mediating the dramatic morphogenetic events that accompany the transition to PH growth. Twelve "alanine scan" alleles of the single yeast actin gene (ACT1) were tested for effects on filamentation, unipolar budding, agar invasion, and cell elongation. Some act1 mutations affect all phenotypes, whereas others affect only one or two aspects of PH growth. Tests of intragenic complementation among specific act1 mutations support the phenotypic evidence for multiple actin functions in filamentous growth. We present evidence that interaction between actin and the actin-binding protein fimbrin is important for PH growth and suggest that association of different actin-binding proteins with actin mediates the multiple functions of actin in filamentous growth. Furthermore, characterization of cytoskeletal structure in wild type and act1/act1 mutants indicates that PH cell morphogenesis requires the maintenance of a highly polarized actin cytoskeleton. Collectively, this work demonstrates that ac...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Sep 6, 1990·Nature·K C HolmesW Kabsch
Jan 1, 1989·Methods in Cell Biology·D A AgardJ W Sedat
Dec 1, 1988·The Journal of Cell Biology·D G DrubinD Botstein
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Jan 1, 1995·Nature Structural Biology·D C AmbergD Botstein
Jan 1, 1995·Molecular and Cellular Biology·A E AdamsP Matsudaira
Sep 1, 1994·Molecular Biology of the Cell·S J KronG R Fink
Jul 1, 1994·The Journal of Cell Biology·D A HoltzmanD G Drubin
Apr 1, 1994·The Journal of Cell Biology·J MulhollandD Botstein
Feb 1, 1994·Current Opinion in Cell Biology·M D WelchD G Drubin
Mar 1, 1993·The Journal of Cell Biology·D J Lew, S I Reed
Mar 1, 1996·The Journal of Cell Biology·J A WaddleJ A Cooper
Apr 1, 1996·Journal of Microscopy·B A ScalettarD A Agard
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Doyle, D Botstein
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K R Ayscough, D G Drubin
Dec 15, 1996·Experientia·H RiezmanL Hicke
Oct 23, 1997·The Journal of Cell Biology·D HaneinD J DeRosier

❮ Previous
Next ❯

Citations

Jan 12, 2001·FEMS Microbiology Reviews·J M Gancedo
Feb 26, 2000·Molecular Biology of the Cell·H Jin, D C Amberg
May 4, 2000·Molecular Biology of the Cell·K L RichardsD Botstein
Nov 6, 2001·Molecular Biology of the Cell·S H AhnS J Kron
Jun 1, 2004·Molecular Biology of the Cell·Ulrich GüldenerJohannes H Hegemann
Nov 29, 2002·Eukaryotic Cell·U OberholzerM Whiteway
Mar 10, 2005·Eukaryotic Cell·Martine BassilanaRobert A Arkowitz
Feb 7, 2001·Molecular and Cellular Biology·C M AslesonJ Berman
Aug 15, 2002·Current Biology : CB·Michael F Olson
Mar 12, 2008·Molecular Microbiology·Srijana Upadhyay, Brian D Shaw
Sep 28, 2017·G3 : Genes - Genomes - Genetics·Jahaun AzadmaneshJill R Blankenship
Jun 20, 2013·Journal of Cell Science·Isabelle GuillasRobert A Arkowitz
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·D I Johnson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.