Multiple functions of yeast mitochondrial transcription factor Mtf1p during initiation.

The Journal of Biological Chemistry
Maria SavkinaMichael Anikin

Abstract

Transcription of the yeast mitochondrial genome is carried out by an RNA polymerase (Rpo41p) that is related to single subunit bacteriophage RNA polymerases but requires an additional factor (Mtf1p) for initiation. In this work we show that Mtf1p is involved in multiple roles during initiation including discrimination of upstream base pairs in the promoter, initial melting of three to four base pairs around the site of transcript initiation, and suppression of nonspecific initiation. It, thus, appears that Mtf1p is functionally analogous to initiation factors of multisubunit RNA polymerases, such as sigma. Photocross-linking experiments reveal close proximity between Mtf1p and the promoter DNA and show that the C-terminal domain makes contacts with the template strand in the vicinity of the start site. Interestingly, Mtf1p is related to a class of RNA methyltransferases, suggesting an early evolutionary link between RNA synthesis and processing.

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Citations

Dec 17, 2009·The Journal of Biological Chemistry·Swaroopa Paratkar, Smita S Patel
Apr 2, 2011·The Journal of Biological Chemistry·Swaroopa ParatkarSmita S Patel
Sep 14, 2011·The Journal of Biological Chemistry·Guo-Qing TangSmita S Patel
Sep 14, 2011·Nucleic Acids Research·Hajin KimTaekjip Ha
Jan 8, 2014·Nucleic Acids Research·Yaroslav I MorozovDmitry Temiakov
Jan 15, 2014·Nucleic Acids Research·Elena YakubovskayaMiguel Garcia-Diaz
Feb 25, 2014·Mitochondrion·José M Gualberto, Kristina Kühn
Feb 23, 2012·Biochimica Et Biophysica Acta·Aishwarya P Deshpande, Smita S Patel
Sep 29, 2011·Nature·Rieke RingelDmitry Temiakov
Aug 29, 2020·Nature Communications·Byeong-Kwon SohnHajin Kim
Nov 1, 2020·The Journal of Biological Chemistry·Urmimala BasuSmita S Patel
Jan 18, 2021·The Journal of Biological Chemistry·Urmimala BasuSmita S Patel

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